Rabbit anti-CHD4/Mi2 beta Antibody, Affinity Purified
SIGMA/PLA0165 - Powered by Bethyl Laboratories, Inc.
Synonym: ATP-dependent helicase CHD4; CHD-4; Mi-2 autoantigen 218 kDa protein; Mi-2b; Mi2-BETA
Product Type: Product-on-demand
Western Blotting Rabbit Anti-CHD4/Mi2 beta Antibody, Affinity Purified: Cat. No. PLA0165: Detection of Human and Mouse CHD4/Mi2 beta by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-CHD4/Mi2 beta antibody Cat. No. PLA0165 used for WB at 0.04 μg/mg. Detection: Chemiluminescence with exposure time of 10 seconds.
Immunoprecipitation Rabbit Anti-CHD4/Mi2 beta Antibody, Affinity Purified: Cat. No. PLA0165: Detection of Human CHD4/Mi2 beta by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-CHD4/Mi2 beta antibody Cat. No. PLA0165 used for WB at 0.1 μg/mL and for IP at 3 μg/mg lysate. CHD4/Mi2 beta was also immunoprecipitated by Rabbit Anti-CHD4/Mi2 beta Antibody B and C, which recognize other epitopes. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_001264.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0165 maps to a region between residue 250 and 300 of human chromodomain helicase DNA binding protein 4 (Mi2 autoantigen 218 kDa protein) using the numbering given in entry NP_001264.2 (GeneID 1108). |
| Other Notes: | CHD4/Mi2beta is a helicase component of the nucleosome remodeling and deacetylase (NuRD) complex that functions to remodel chromatin and repress transcription. Outside of the NuRD complex, CHD4/Mi2beta can function as an activator of transcription in association with p300 histone acetyltransferase. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |