Rabbit anti-TRRAP Antibody, Affinity Purified
SIGMA/PLA0167 - Powered by Bethyl Laboratories, Inc.
Synonym: 350/400 kDa PCAF-associated factor; PAF350/400; PAF400; STAF40; TR-AP; Tra1; tra1 homolog
Product Type: Product-on-demand
Western Blotting Rabbit Anti-TRRAP Antibody, Affinity Purified: Cat. No. PLA0167: Detection of Human and Mouse TRRAP by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-TRRAP antibody Cat. No. PLA0167 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-TRRAP Antibody, Affinity Purified: Cat. No. PLA0167: Detection of Human TRRAP by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-TRRAP antibody Cat. No. PLA0167 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. TRRAP was also immunoprecipitated by Rabbit Anti-TRRAP Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | AAD04629.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q9Y4A5  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0167 maps to a region between residue 3809 and 3859 of human transformation/transcript |
| Other Notes: | TRRAP is a common component of histone acetyltransferase (HAT) complexes and functions as an adaptor protein that recruits HAT complexes to chromatin during transcription, replication, and DNA repair. TRRAP has been shown to play a key role in c-Myc-mediated transcription and transformation. TRAPP has also been observed to interact with many other transcription factors including p53, E2F1, E2F4, and the E1A viral oncoprotein. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |