Rabbit anti-NCoR Antibody, Affinity Purified
SIGMA/PLA0170
Synonym: KIAA1047; MGC104216; N-CoR; N-CoR1; PPP1R109; TRAC1; hCIT529I10; hN-CoR; nuclear receptor co-repressor 1; protein phosphatase 1; regulatory subunit 109; thyroid hormone- and retinoic acid receptor-associated corepressor 1
Product Type: Product-on-demand
Western Blotting Rabbit Anti-NCoR Antibody, Affinity Purified: Cat. No. PLA0170: Detection of Human NCOR by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-NCOR antibody Cat. No. PLA0170 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Western Blotting Rabbit Anti-NCoR Antibody, Affinity Purified: Cat. No. PLA0170: Detection of Mouse NCOR by Western Blot. Samples : Whole cell lysate (50 μg) from TCMK-1, Renca, 4T1, and CT26.WT cells. Antibodies: Affinity purified Rabbit Anti-NCOR Antibody PLA0170 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-NCoR Antibody, Affinity Purified: Cat. No. PLA0170: Detection of Human NCOR by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-NCOR antibody Cat. No. PLA0170 used for WB at 1.0 μg/mL and for IP at 3 μg/mg lysate. NCOR was also immunoprecipitated by Rabbit Anti-NCOR Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 3 seconds.
| accession no. | NP_006302.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| proximity ligation assay: 10 μg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0170 maps to a region between residue 1525 and 1575 of human nuclear receptor co-repressor 1 using the numbering given in entry NP_006302.2 (GeneID 9611). |
| Other Notes: | The nuclear Receptor corepressor protein (NCoR) mediates gene repression by interacting with nuclear receptors such as the thyroid hormone receptor, retinoic acid receptor, progesterone receptor, androgen receptor, estrogen receptor, and glucocorticoids receptor. NCoR mediates repression by associating with SIN3A/B and the histone deacetylases, HDAC1 and HDAC2, and promoting the formation of repressive chromatin structures. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |