Rabbit anti-PML Antibody, Affinity Purified
SIGMA/PLA0172 - Powered by Bethyl Laboratories, Inc.
Synonym: MYL; PML/RARA fusion; PP8675; RING finger protein 71; RNF71; TRIM19; inducer of; probable transcription factor PML; promyelocytic leukemia; promyelocytic leukemia protein; tripartite motif protein TRIM19; tripartite motif-containing protein 19
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-PML Antibody, Affinity Purified: Cat. No. PLA0172: Detection of Human PML by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-PML (Cat. No. PLA0172) used at a dilution of 1:1,000 (0.2 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-PML Antibody, Affinity Purified: Cat. No. PLA0172: Detection of Human PML by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 μg), 293T (Lane T; 50 μg) and Jurkat (Lane J; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-PML antibody Cat. No. PLA0172 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 30 second.
Immunoprecipitation Detection of Human PML by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-PML antibody Cat. No. PLA0171 used for WB at 0.4 μg/mL and for IP at 6 μg/mg lysate. PML was also immunoprecipitated by PML Antibody B. Detection: Chemiluminescence with exposure time of 3 seconds.
| accession no. | NP_150241.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:1,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P29590  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0172 maps to a region between residue 375 and 425 of promyelocytic leukemia using the numbering given in entry NP_150241.2 (GeneID 5371). |
| Other Notes: | PML is a member of RBCC/TRIM family of proteins that possess a RING finger domain, B-box, and coiled-coil domain. PML is a multifunctional protein involved in activities such as tumor growth suppression, hematopoietic differentiation, gene transcription, apoptotic induction, and viral immunity. PML is often involved in chromosomal translocations with the retinoic acid receptor alpha gene. PML-retinoic acid receptor translocations are associated with acute promyelocytic leukemia. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |