Rabbit anti-BRF1 Antibody, Affinity Purified
SIGMA/PLA0175 - Powered by Bethyl Laboratories, Inc.
Synonym: 90kD; 90kD subunit; B - related factor 1; B-related factor 1; BRF; BRF-1; BRF1 homolog; BRF1 homolog; CFDS; GTF3B; GTF3B subunit 2; HEL-S-76p; RNA polymerase III; RNA polymerase III; RNA polymerase III; TAF3B2; TAF3C; TAFIII90; TATA box binding protein (TBP)-associated factor; TATA box binding protein (TBP)-associated factor 3C; TATA box-binding protein-associated factor; TBP - associated factor; TF3B90; TFIIIB90; epididymis secretory sperm binding protein Li 76p; general transcription factor IIIB; hBRF; hTFIIIB90; subunit 2; subunit of RNA polymerase III transcription initiation factor IIIB; subunit of RNA polymerase III transcription initiation factor IIIB (S. cerevisiae)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-BRF1 Antibody, Affinity Purified: Cat. No. PLA0175: Detection of Human BRF1 by Immunohistochemistry. Sample: FFPE section of Human thyroid carcinoma. Antibody: Affinity purified Rabbit Anti-BRF1 (Cat. No. PLA0175) used at a dilution of 1:200 (1 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-BRF1 Antibody, Affinity Purified: Cat. No. PLA0175: Detection of Human and Mouse BRF1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-BRF1 antibody Cat. No. PLA0175 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 10 seconds.
Immunoprecipitation Rabbit Anti-BRF1 Antibody, Affinity Purified: Cat. No. PLA0175: Detection of Human BRF1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa celss (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-BRF1 antibody Cat. No. PLA0175 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. BRF1 was also immunoprecipitated by Rabbit Anti-BRF1 Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 3 seconds.
| accession no. | NP_001510.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:1,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q92994  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0175 maps to a region between residue 627 and 677 of human B - related factor 1 using the numbering given in entry NP_001510.2 (GeneID 2972). |
| Other Notes: | BRF1 is one of three subunits of the RNA polymerase III transcription factor complex, TFIIIB. TFIIIB regulates RNA Polymerase III transcription which manufactures tRNAs, 5S ribosomal RNA and several other untranslated RNAs. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |