Rabbit anti-NBS1 Antibody, Affinity Purified
SIGMA/PLA0179 - Powered by Bethyl Laboratories, Inc.
Synonym: AT-V1; AT-V2; ATV; NBS; NBS1; Nijmegen breakage syndrome 1 (nibrin); Nijmegen breakage syndrome protein 1; P95; cell cycle regulatory protein p95; p95 protein of the MRE11/RAD50 complex
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-NBS1 Antibody, Affinity Purified: Cat. No. PLA0179: Detection of Human NBS1 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-NBS1 (Cat. No. PLA0179) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-NBS1 Antibody, Affinity Purified: Cat. No. PLA0179: Detection of Human NBS1 by Western Blot. Samples: Whole cell lysate from HeLa cells (5, 15 and 50 μg). Antibodies: Affinity purified Rabbit Anti-NBS1 antibody Cat. No. PLA0179 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
Immunoprecipitation Rabbit Anti-NBS1 Antibody, Affinity Purified: Cat. No. PLA0179: Detection of Human NBS1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-NBS1 antibody PLA0179 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. NBS1 was also immunoprecipitated by Rabbit Anti-NBS1 Antibody B and C, which recognize upstream epitopes. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_002476.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100- 1:500 |
| immunoprecipitation (IP): 2- 5 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0179 maps to a region between residue 704 and 754 of human Nibrin using the numbering given in entry NP_002476.2 (GeneID 4683). |
| Other Notes: | Mutations in NBS1 (Nijmegen Breakage Syndrome 1), also known as nibrin, are associated with the autosomal recessive syndrome, Nijmegen breakage syndrome, characterized by microcephaly, growth retardation, immunodeficiency, and cancer predisposition. At the cellular level, NBS1 is part of the MRE11/RAD50 double-strand break repair complex that is critical to the DNA damage response, DNA recombination, telomere integrity, and cell cycle checkpoint control. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |