Rabbit anti-PSF/SFPQ Antibody, Affinity Purified
SIGMA/PLA0181 - Powered by Bethyl Laboratories, Inc.
Synonym: 100 kDa DNA-pairing protein; 100 kDa subunit; DNA-binding p52/p100 complex; POMP100; PPP1R140; PSF; PTB-associated splicing factor; PTB-
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-PSF/SFPQ Antibody, Affinity Purified: Cat. No. PLA0181: Detection of Human PSF/SFPQ by Immunohistochemistry. Sample: FFPE section of Human colon carcinoma. Antibody: Affinity purified Rabbit Anti-PSF/SFPQ (Cat. No. PLA0181) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-PSF/SFPQ Antibody, Affinity Purified: Cat. No. PLA0181: Detection of Human and Mouse PSF/SFPQ by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-PSF/SFPQ antibody Cat. No. PLA0181 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 3 seconds.
Immunoprecipitation Rabbit Anti-PSF/SFPQ Antibody, Affinity Purified: Cat. No. PLA0181: Detection of Human PSF/SFPQ by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-PSF/SFPQ antibody Cat. No. PLA0181 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. PSF/SFPQ was also immunoprecipitated by Rabbit Anti-PSF/SFPQ Sntibody B and C, which recognize other epitopes. Detection: Chemiluminescence with exposure time of 1 second.
| accession no. | NP_005057.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100- 1:500 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0181 maps to a region between residue 225 and 275 of human Polypyrimidine tract-binding protein-associated-splici |
| Other Notes: | PSF/SFPQ is a multifunctional protein know to participate in a variety of nuclear processes and may function to link transcription and pre-mRNA processing. PSF/SFPQ bears an RNA recognition motif (RRM) further indicating its role in post-transcriptional processes. PSF has been shown to associate with p54nrb/NonO to modulate androgen receptor-mediated transcription. Additionally, it has recently been show to associate with XRN2 and p54nrb/NonO to facilitate pre-mRNA 3’ processing and transcription termination. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |