Rabbit anti-BRCA1 Antibody, Affinity Purified
SIGMA/PLA0185 - Powered by Bethyl Laboratories, Inc.
Synonym: BRCA1/
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-BRCA1 Antibody, Affinity Purified: Cat. No. PLA0185: Detection of Human BRCA1 by Immunohistochemistry. Samples: FFPE section of Human breast carcinoma (left) and ovarian carcinoma (right). Antibody: Affinity purified Rabbit Anti-BRCA1 (Cat. No. PLA0185) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-BRCA1 Antibody, Affinity Purified: Cat. No. PLA0185: Detection of Human BRCA1 by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRCA1 Antibody PLA0185 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-BRCA1 Antibody, Affinity Purified: Cat. No. PLA0185: Detection of Human BRCA1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRCA1 antibody Cat. No. PLA0185 used for IP at 6 μg/mg lysate. BRCA1 was also immunoprecipitated by Rabbit Anti-BRCA1 antibody B. For blotting immunoprecipitated BRCA1, PLA0185 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_009225.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:500- 1:2,500 | |
| UniProt accession no. | P38398  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0185 maps to a region between residue 250 and 300 of human breast cancer 1 using the numbering given in entry NP_009225.1 (GeneID 672). |
| Other Notes: | BRCA1 was identified as a gene with germline mutations that predisposes women to breast and ovarian cancer. The BRCA1 tumor suppressor is a large protein that has been demonstrated to interact with a multitude of proteins involved in the DNA damage response and cell cycle checkpoints to maintain genomic integrity. BRCA1 functions by regulating the activities of its interacting proteins which mediate processes such as gene transcription, gene repression, protein phosphorylation, and ubiquitination. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |