Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified
SIGMA/PLA0201 - Powered by Bethyl Laboratories, Inc.
Synonym: 14-3-3 sigma; Stratifin; YWHAS; epithelial cell marker protein 1
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-14-3-3 Sigma Antibody, Affinity Purified: Cat. No. PLA0201: Detection of Human 14-3-3 Sigma by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-14-3-3 Sigma (Cat. No. PLA0201) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-14-3-3 Sigma Antibody, Affinity Purified: PLA0201: Detection of Human 14-3-3 Sigma by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-14-3-3 Sigma antibody Cat. No. PLA0201 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-14-3-3 Sigma Antibody, Affinity Purified: PLA0201: Detection of Human 14-3-3 Sigma by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-14-3-3 Sigma antibody Cat. No. PLA0201 used for WB at 1 μg/mL and for IP at 6 μg/mg lysate. 14-3-3 Sigma was also immunoprecipitated by Rabbit Anti-14-3-3 Sigma Antibody B. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_006133.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:2,000-1:10,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Application: | Rabbit anti-14-3-3 Sigma Antibody, Affinity Purified has been used in the pull down assay for Rho associated coiled-coil containing protein kinase 1 (ROCK1) proteins and heat shock 70 kDa 4 protein (Hsp74) in HCT116 cancer cells. |
| Biochem/physiol Actions: | Rabbit anti-14-3-3 or stratifin participates in several biological events including apoptosis and cell differentiation. It regulates the transcription matrix metallopeptidases (MMPs) in fibroblasts and may serve as therapeutic potential in wound healing. 14-3-3σ is less expressed and is hyper methylated in breast cancer cells. It plays a key role in linking signalling proteins and in the enhancing of protein kinase C functionality. A mutation in the stratifin gene is implicated in epilation phenotype in mice. It interacts with ubiquitin-specific protease 8 (USP8) and is highly expressed in human lung adenocarcinoma. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Rabbit anti-14-3-3 Sigma or stratifin is mapped to human chromosome 1p36.11. 14-3-3σ protein is expressed as seven isoforms and has molecular weight in the range of 25 to 30 kDa. It is an acidic homodimeric protein and harbors pleckstrin homology (PH) domain. 14-3-3σ is highly expressed in epidermis. |
| Immunogen: | The epitope recognized by PLA0201 maps to a region between residue 198 and 248 of human 14-3-3 sigma using the numbering given in entry NP_006133.1 (GeneID 2810). |
| Other Notes: | 14-3-3 sigma was identified as an epithelial cell marker. It is part of the 14-3-3 family of proteins in which seven isoforms have been identified: beta, zeta, gamma, eta, epsilon, tau, and sigma. 14-3-3 proteins function as adaptors that bind with a number of partners to mediate various signaling pathways. 14-3-3 sigma is also called stratifin or HME1 and appears to function as a tumor suppressor whose expression can be downregulated via methylation. Loss of 14-3-3 sigma expression results in a defective G2/M phase checkpoint and appears to contribute to both epithelial and non-epithelial tumorigenesis. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |