Rabbit anti-BCAR3 Antibody, Affinity Purified
SIGMA/PLA0206 - Powered by Bethyl Laboratories, Inc.
Synonym: NSP2; SH2 domain-containing protein 3B; SH2D3B; breast cancer antiestrogen resistance 3 protein; dJ1033H22.2 (breast cancer anti-estrogen resistance 3); novel SH2-containing protein 2
Product Type: Product-on-demand
Western Blotting Rabbit Anti-BCAR3 Antibody, Affinity Purified: Cat. No. PLA0206: Detection of Human BCAR3 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg) and 293T (T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-BCAR3 antibody Cat. No. PLA0206 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-BCAR3 Antibody, Affinity Purified: Cat. No. PLA0206: Detection of Human BCAR3 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-BCAR3 antibody Cat. No. PLA0206 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. BCAR3 was also immunoprecipitated by Rabbit Anti-BCAR3 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_003558.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 0.2 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | O75815  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0206 maps to a region between residue 25 and 75 of human breast cancer anti-estrogen resistance 3 using the numbering given in entry NP_003558.1 (GeneID 8412). |
| Other Notes: | BCAR3 (breast cancer anti-estrogen resistance 3) was originally identified as a factor that confers resistance to anti-estrogens in breast cancer cells. BCAR3 contains a Ras-GEF domain and an SH2 domain. It is related to SHEP1 and NSP1 and is thought to also play a role as an adaptor protein that mediates integrin signaling to modulate cell adhesion and migration. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |