Rabbit anti-CDK2 Antibody, Affinity Purified
SIGMA/PLA0213 - Powered by Bethyl Laboratories, Inc.
Synonym: CDKN2; cdc2-related protein kinase; cell division protein kinase 2; p33 protein kinase; p33(CDK2)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-CDK2 Antibody, Affinity Purified: Cat. No. PLA0213: Detection of Human and Mouse CDK2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse renal cell carcinoma (right). Antibody: Affinity purified Rabbit Anti-CDK2 (Cat. No. PLA0213) used at a dilution of 1:1,000 (1 μg/mL) and 1:5,000 (0.2 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-CDK2 Antibody, Affinity Purified: Cat. No. PLA0213: Detection of Human CDK2 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg) and 293T (T; 50 μg) cells. Antibody: Affinity purified Rabbit Anti-CDK2 antibody Cat. No. PLA0213 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes .
Immunoprecipitation Rabbit Anti-CDK2 Antibody, Affinity Purified: Cat. No. PLA0213: Detection of Human CDK2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa celss (1 mg for IP, 20% of IP loaded). Antibody: Affinity purified Rabbit Anti-CDK2 antibody Cat. No. PLA0213 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. CDK2 was also immunoprecipitated, albeit less efficiently, by Rabbit Anti-CDK2 Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_001789.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P24941  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0213 maps to a region between residue 248 and 298 of human cyclin-dependent kinase 2 using the numbering given in entry NP_001789.2 (GeneID 1017). |
| Other Notes: | Cyclin-dependent kinase 2 (CDK2) is a member of the CDK family of protein kinases that regulate cell cycle progression. CDK2 associates with cyclin E or cyclin A to drive the cell cycle through G1- and S-phase. Substrates of CDK2 include pRb, p53, p27, E2F, Brca1, CDK7, B-Myb, PP1 alpha, RNA Pol II CTD, axin, p220 NPAT, ribonucleotide reductase M2, Myo-D, MARCKS, HsCdc6, and CP110. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |