Rabbit anti-NF-kappaB2 Antibody, Affinity Purified
SIGMA/PLA0215 - Powered by Bethyl Laboratories, Inc.
Synonym: CVID10; DNA-binding factor KBF2; H2TF1; LYT-10; LYT10; NF-kB2; NFKB; Nuclear factor of kappa light polypeptide gene enhancer in B-cells 2; lymphocyte translocation chromosome 10 protein; nuclear factor Kappa-B; nuclear factor NF-kappa-B p52 subunit; nuclear factor of Kappa light chain gene enhancer in B cells 2; nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100); oncogene Lyt-10; p100; p105; p49/p100; p52; p52/p100 subunit; subunit 2; transcription factor NFKB2
Product Type: Product-on-demand
Western Blotting Rabbit Anti-NF-kappaB2 Antibody, Affinity Purified: Cat. No. PLA0215: Detection of Human and Mouse NF-kappaB2 by Western Blot. Samples: Whole cell lysate from HeLa (H; 50 μg), Jurkat (J; 50 μg), MCF7 (M; 50 μg), 293T (T; 50 μg), and Mouse NIH3T3 (3; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-NF-kappaB2 antibody Cat. No. PLA0215 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation Detection of Human by Nf-kappaB2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from Jurkat cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-NF-kappaB2 antibody Cat. No. PLA0215 used for WB at 0.4 μg/mL and for IP at 3 μg/mg lysate. NF-kappaB2 was also immunoprecipitated by Rabbit Anti-NF-kappaB2 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 3 minutes.
| accession no. | NP_001070962.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0215 maps to a region between residue 810 and 860 of human nuclear factor of kappa light polypeptide gene enhancer in B-cells 2 (p49/p100) using the numbering given in entry NP_001070962.1 (GeneID 4791). |
| Other Notes: | The NFKB2 gene encodes a 100 kDa protein that can undergo co-translational processing to also yield a 52 kDa protein. The 52 kDa protein is also known as NF-kappa B2/p52 and is one of five monomeric subunits that are part of the Rel family of proteins that dimerize to form the NF-kappa B (nuclear factor that binds the kappa immunoglobulin light chain gene enhancer) transcription factor. Members of the Rel family of proteins include NF-kappa B1/p50, NF-kappa B2/p52, RelA/p65, c-Rel, and RelB. The 100 kDa product is also known as p100 or I kappa B delta. P100/I kappa B delta has been shown to function as an inhibitor protein that interacts with NF-kappa B and inhibit its DNA binding activity. P100/I kappa B delta is a member of the I kappa B family of proteins which includes I kappa B-beta, I kappa B-delta/NF-kappa B2/p100, I kappa B-gamma/NF-kappa B1/p105, I kappa B-epsilon, I kappa B-zeta, and Bcl3. The classical description of the NF-kappa B transcription factor is a transcription factor that reprograms gene expression of the immune system in response to injury, infection, and stress. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |