Rabbit anti-ORC1 Antibody, Affinity Purified
SIGMA/PLA0221 - Powered by Bethyl Laboratories, Inc.
Synonym: HSORC1; ORC1L; PARC1; S. cerevisiae; homolog-like; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex 1; replication control protein 1; subunit 1; subunit 1; subunit 1 (yeast homolog)-like; subunit 1 homolog; subunit 1 homolog (S. cerevisiae); subunit 1-like (yeast)
Product Type: Product-on-demand
Western Blotting Rabbit Anti-ORC1 Antibody, Affinity Purified: Cat. No. PLA0221: Detection of Human ORC1 by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 μg), 293T (T; 50 μg) and Jurkat (J; 50 mcg) cells. Antibodies: Affinity purified Rabbit Anti-ORC1 antibody Cat. No. PLA0221 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-ORC1 Antibody, Affinity Purified: Cat. No. PLA0221: Detection of Human ORC1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-ORC1 antibody Cat. No. PLA0221 used for WB at 1 μg/mL and for IP at 6 μg/mg lysate. ORC1 was also immunoprecipitated by Rabbit Anti-ORC1 Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_004144.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q13415  |
| Application: | Rabbit anti-ORC1 Antibody, Affinity Purified has been used in immunoblotting (1:2,000). |
| Biochem/physiol Actions: | The origin recognition complex (ORC) plays a key role in DNA replication. ORC1 is degraded in the S phase and re-synthesized in the G1 phase of the cell cycle. ORC1 is responsible for cell proliferation, apoptosis, invasion, and migration. ORC1 regulates cyclin E1 (CCNE1) gene expression and is involved in genomic maintenance. Downregulation of ORC1 and cyclins cause Meier–Gorlin syndrome. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Origin recognition complex 1 (ORC1) is the largest subunit of ORC. The subunits are widely expressed in the human lung, bones, kidney, and uterus. The ORC1 gene is mapped to human chromosome 1p32.3. |
| Immunogen: | The epitope recognized by PLA0221 maps to a region between residue 811 and 861 of human origin recognition complex subunit 1 using the numbering given in entry NP_004144.2 (GeneID 4998). |
| Other Notes: | ORC1 (origin recognition complex, subunit 1) is one of six subunits that make up the origin of recognition complex (ORC). The ORC serves as a scaffold for proteins involved in the initiation of DNA replication. Very recently, ORC1 has been found to perform a function independent of its role in DNA replication. In a screen using siRNAs for human ORC proteins to assess their roles in centrosome biology, it was found that ORC1 plays a key role in controlling centriole and centrosome copy number in human cells. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |