Rabbit anti-IKK-alpha Antibody, Affinity Purified
SIGMA/PLA0224 - Powered by Bethyl Laboratories, Inc.
Synonym: Conserved helix-loop-helix ubiquitous kinase; I-kappa-B kinase 1; I-kappa-B kinase alpha; I-kappa-B kinase-alpha; IKK-A; IKK-a kinase; IKK-alpha; IKK1; IKKA; IkB kinase alpha subunit; IkBKA; IkappaB kinase; NFKBIKA; Nuclear factor NF-kappa-B inhibitor kinase alpha; Nuclear factor NFkappaB inhibitor kinase alpha; TCF-16; TCF16; transcription factor 16
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-IKK-alpha Antibody, Affinity Purified: Cat. No. PLA0224: Detection of Human and Mouse IKK-alpha by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma (left) and Mouse teratoma (right). Antibody: Affinity purified Rabbit Anti-IKK-alpha (Cat. No. PLA0224) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-IKK-alpha Antibody, Affinity Purified: Cat. No. PLA0224: Detection of Human IKK-alpha by Western Blot. Samples: Whole cell lysate (5, 15 and 50 μg) from Jurkat cells. Antibodies: Affinity purified Rabbit Anti-IKK-alpha antibody Cat. No. PLA0224 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-IKK-alpha Antibody, Affinity Purified: Cat. No. PLA0224: Detection of Human IKK-alpha by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from Jurkat cells. Antibodies: Affinity purified Rabbit Anti-IKK-alpha antibody Cat. No. PLA0224 used for WB at 1 μg/mL and used for IP at 3 μg/mg lysate. IKK-alpha was also immunoprecipitated by Rabbit Anti-IKK-alpha Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_001269.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 500  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100- 1:500 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0224 maps to a region between residue 350 and 400 of human I-kappa-B kinase-alpha using the numbering given in entry NP_001269.3 (GeneID 1147). |
| Other Notes: | I kappa-B kinase-alpha (IKK-alpha) is part of the IKK complex composed of two regulatory subunits, IKK-alpha and IKK-beta, and a regulatory subunit, IKK-gamma/NEMO. The IKK complex functions to phosphorylate and inactivate I kappa B, an inhibitor of the NF-kappa B transcription factor. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |