Rabbit anti-CKII beta Antibody, Affinity Purified
SIGMA/PLA0226 - Powered by Bethyl Laboratories, Inc.
Synonym: CK II beta; CK2B; CK2N; CSK2B; Casein kinase II beta subunit; G5A; G5a; beta polypeptide; casein kinase 2; phosvitin; phosvitin; protein G5a
Product Type: Product-on-demand
Western Blotting Rabbit Anti-CKII beta Antibody, Affinity Purified: Cat. No. PLA0226: Detection of Human and Mouse CKII beta by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (T; 50 μg) and Mouse NIH3T3 (M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-CKII beta antibody Cat. No. PLA0226 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-CKII beta Antibody, Affinity Purified: Cat. No. PLA0226: Detection of Human CKII beta by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-CKII beta antibody Cat. No. PLA0226 used for WB at 1 μg/mL and used for IP at 3 μg/mg lysate. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_001311.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0226 maps to a region between residue 165 and 215 of human casein kinase 2, beta using the numbering given in entry NP_001311.3 (GeneID 1460). |
| Other Notes: | CKII beta (casein kinase II subunit beta) is the beta subunit of the heterotetrameric kinase, casein kinase II (CKII) which is composed of an alpha subunit, an alpha’ subunit, and two beta subunits. CKII localizes to the endoplasmic reticulum and the Golgi apparatus and is involved in signal transduction and cell cycle progression. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |