Rabbit anti-BRD4 Antibody, Affinity Purified
SIGMA/PLA0227 - Powered by Bethyl Laboratories, Inc.
Synonym: CAP; HUNK1; HUNKI; MCAP; Protein HUNK1; bromodomain-
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Mouse BRD4 by Immunohistochemistry. Samples: FFPE sections of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-BRD4 (Cat. No. PLA0227) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.
Immunohistochemistry Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Human BRD4 by Immunohistochemistry. Samples: FFPE sections of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-BRD4 (Cat. No. PLA0227) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Human BRD4 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa, 293T, and Jurkat cells. Antibodies: Affinity purified Rabbit Anti-BRD4 Antibody (Cat. No. PLA0227) used for WB at 0.04 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-BRD4 Antibody, Affinity Purified: Cat. No. PLA0227: Detection of Human BRD4 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-BRD4 antibody Cat. No. PLA0227 used for IP at 6 μg/mg lysate. BRD4 was also immunoprecipitated by Rabbit Anti-BRD4 Antibody B. For blotting immunoprecipitated BRD4, PLA0227 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 seconds.
| accession no. | NP_490597.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1,000-1:5,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | O60885  |
| Biochem/physiol Actions: | Bromodomain-containing protein 4 (BRD4) works as a reader of epigenetic marks and is involved in chromatin remodeling and transcriptional regulation. Therefore, it is a target for oncology, inflammation and cardiovascular disease. This gene is upregulated in hepatocellular carcinoma and is associated with poor prognosis. BRD4 is also associated with melanoma, hepatocellular carcinoma, multiple myeloma, Burkitt′s lymphoma, acute myeloid leukemia and breast cancer. BRD4 connects cell cycle and transcription, works as a scaffold for transcription factors and bookmarks active genes during mitosis. It interacts with acetylated histone-3 and histone-4 tails and helps in maintaining chromatin architecture. Also, it regulates transcription elongation via phosphorylation of RNA polymerase II. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Bromodomain-containing protein 4 (BRD4) belongs to the bromodomain and extra-terminal (BET) family. The protein has two bromodomains (BD1 and BD2) connected by a long interdomain linker. |
| Immunogen: | The epitope recognized by PLA0227 maps to a region between residue 1312 and 1362 of human bromodomain-containing protein 4 using the numbering given in entry NP_490597.1 (GeneID 23476). |
| Other Notes: | Bromodomain-containing protein 4 (BRD4) contains 2 bromo-domains. Bromo-domains are found in chromatin-associating proteins and can interact specifically with acetylated lysine. BRD4 is described as a chromatin adaptor and has been shown to bind acetylated chromatin. It was originally identified as MCAP (mitotic chromosome associated protein) due to its association with mitotic chromosomes. BRD4 also associates with several transcription complexes such as the Mediator complexes and P-TEFb complexes and is considered to function as a transcriptional co-factor. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |