Rabbit anti-G3BP1 Antibody, Affinity Purified
SIGMA/PLA0231 - Powered by Bethyl Laboratories, Inc.
Synonym: ATP-dependent DNA helicase VIII; DNA helicase VIII; G3BP; G3BP-1; GAP SH3 domain-binding protein 1; GAP binding protein; GTPase activating protein (SH3 domain) binding protein 1; HDH-VIII; Ras-
Product Type: Product-on-demand
Western Blotting Rabbit Anti-G3BP1 Antibody, Affinity Purified: Cat. No. PLA0231: Detection of Human and Mouse G3BP1 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (T; 50 μg) and Mouse NIH3T3 (M; 50μg) cells. Antibodies: Affinity purified Rabbit Anti-G3BP1 antibody Cat. No. PLA0231 used for WB at 0.04 μg/m. Detection: Chemiluminescence with exposure time of 10 seconds.
Immunoprecipitation Rabbit Anti-G3BP1 Antibody, Affinity Purified: Cat. No. PLA0231: Detection of Human G3BP1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-G3BP1 antibody Cat. No. PLA0231 used for WB at 1 μg/mL and for IP at 3 μg/mg lysate. G3BP1 was also immunoprecipitated by Rabbit Anti-G3BP1 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 3 seconds.
| accession no. | NP_005745.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q13283  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0231 maps to a region between residue 275 and 325 of human GTPase activating protein (SH3 domain) binding protein 1 using the numbering given in entry NP_005745.1 (GeneID 10146). |
| Other Notes: | Ras-GAP SH3 domain binding protein (G3BP1) contains an RNA recognition motif (RRM) and an N-terminal nuclear transport factor 2-like (NTF2-like) domain. It was first identified in a screen for proteins that bind the SH3 domain of the Ras GTPase activating protein (RasGAP). It has been shown to function downstream of Ras and play a role in RNA metabolism, signal transduction, and proliferation. G3BP1 is primarily cytoplasmic but has also been localized to the nucleus. G3BP1 has been shown to be overexpressed in human cancers. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |