Rabbit anti-GSK3-beta Antibody, Affinity Purified
SIGMA/PLA0232 - Powered by Bethyl Laboratories, Inc.
Synonym: GSK-3 beta; GSK3beta isoform; serine/
Product Type: Product-on-demand
Western Blotting Rabbit Anti-GSK3-beta Antibody, Affinity Purified: Cat. No. PLA0232: Detection of Human and Mouse GSK3-beta by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (T; 50 μg) and Mouse NIH3T3 (M; 50μg) cells. Antibodies: Affinity purified Rabbit Anti-GSK3-beta antibody Cat. No. PLA0232 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-GSK3-beta Antibody, Affinity Purified: Cat. No. PLA0232: Detection of Human GSK3-beta by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-GSK3-beta antibody Cat. No. PLA0232 used for WB at 1 μg/mL and for IP at 10 μg/mg lysate. GSK3-beta was also immunoprecipitated by Rabbit Anti-GSK3-beta Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | AAA66475.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 10 μg/mg |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0232 maps to a region between residue 1 and 50 of human glycogen synthase kinase-3 beta using the numbering given in entry AAA66475.1 (GeneID 2932). |
| Other Notes: | Glycogen synthase kinase 3 beta (GSK3beta) is a proline-directed serine-threonine kinase identified as a kinase responsible for the phosphorylation and inactivation of glycogen synthase. GSK3beta is a critical component of the Wnt/beta-catenin signaling pathway, and its activity results in the inhibition of Wnt signaling by mediating the degradation of beta-catenin. In addition to the Wnt-signaling pathway, GSK3beta is also involved in the Hedgehog, Notch and insulin signaling pathways. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |