Rabbit anti-ERK2 Antibody, Affinity Purified
SIGMA/PLA0236 - Powered by Bethyl Laboratories, Inc.
Synonym: ERK; ERK-2; ERK2; ERT1; MAP kinase 1; MAP kinase 2; MAP kinase isoform p42; MAPK 1; MAPK 2; MAPK2; P42MAPK; PRKM1; PRKM2; extracellular signal-regulated kinase 2; mitogen-activated protein kinase 2; p38; p40; p41; p41mapk; p42-MAPK; protein tyrosine kinase ERK2
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-ERK2 Antibody, Affinity Purified: Cat. No. PLA0236: Detection of Human ERK2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-ERK2 (Cat. No. PLA0236) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-ERK2 Antibody, Affinity Purified: Cat. No. PLA0236: Detection of Human and Mouse ERK2 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg), 293T (Lane T; 50 μg) and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibody: Affinity purified Rabbit Anti-ERK2 Antibody PLA0236 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_002736.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0236 maps to a region between residue 310 and 360 of human extracellular signal-regulated kinase-2 (mitogen-activated protein kinase 1) using the numbering given in entry NP_002736.3 (GeneID 5594). |
| Other Notes: | Extracellular signal-regulated kinase 2 (ERK2/p42) is also known as mitogen-activated protein kinase 1 (MAPK1). ERK2 is part of a signal transduction pathway that begins with the small GTPase Ras and continues through a cascade of protein kinases that include Raf and MEK. Activation of this signaling pathway influences many cellular processes such as proliferation, differentiation and survival. ERK2 is very similar in sequence to ERK1/p44 and the activities of both ERK1 and ERK2 are usually considered together as total ERK activity. More recent evidence has shown that ERK1 and ERK2 are not functionally redundant and may have very different and specific roles. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |