Rabbit anti-AKT1 Antibody, Affinity Purified
SIGMA/PLA0238 - Powered by Bethyl Laboratories, Inc.
Synonym: AKT; AKT1m; CWS6; PKB; PKB alpha; PKB-ALPHA; PRKBA; Protein kinase B; RAC; RAC-ALPHA; RAC-PK-alpha; protein kinase B alpha; proto-oncogene c-Akt; rac protein kinase alpha; serine-threonine protein kinase; v-akt murine thymoma viral oncogene homolog 1; v-akt murine thymoma viral oncogene-like protein 1
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-AKT1 Antibody, Affinity Purified: Cat. No. PLA0238: Detection of Human AKT1 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-AKT1 (Cat. No. PLA0238) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-AKT1 Antibody, Affinity Purified: Cat. No. PLA0238: Detection of Human AKT1 by Western Blot. Samples: Whole cell lysate (50 μg) from 293T (T), MCF7 (M), Jurkat (J) and PANC1 (P) cells. Antibody: Affinity purified Rabbit Anti-AKT1 Antibody PLA0238 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds.
| accession no. | NP_005154.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500-1:2,000 |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P31749  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0238 maps to a region between residue 25 and 75 of human v-akt murine thymoma viral oncogene homolog 1 using the numbering given in entry NP_005154.1 (GeneID 207). |
| Other Notes: | AKT1 is a serine-threonine kinase that functions downstream of PI-3 kinase and is known to phosphorylate several proteins. AKT1 mediates the effects of several growth factors such as insulin, IGF-1, EGF, and PDGF to control cell survival and apoptosis. There are numerous substrates of AKT1, some of which are: GSK3-alpha and beta, mTOR, TSC2, Bad, c-Raf, and caspase 9. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |