Rabbit anti-FANCD2 Antibody, Affinity Purified
SIGMA/PLA0243 - Powered by Bethyl Laboratories, Inc.
Synonym: FA-D2; FA4; FACD; FAD; FAD2; FANCD; Fanconi anemia; Protein FACD2; complementation group D2
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-FANCD2 Antibody, Affinity Purified: Cat. No. PLA0243: Detection of Human FANCD2 by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-FANCD2 (Cat. No. PLA0243) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-FANCD2 Antibody, Affinity Purified: Cat. No. PLA0243: Detection of Human FANCD2 by Western Blot. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg) and 293T (T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-FANCD2 antibody Cat. No. PLA0243 used for WB at 0.04 μg/mL. Detection: Chemiluminescence with exposure time of 3 minutes.
Immunoprecipitation Rabbit Anti-FANCD2 Antibody, Affinity Purified: Cat. No. PLA0243: Detection of Human FANCD2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from 293T cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-FANCD2 antibody Cat. No. PLA0243 used for WB at 1 μg/mL and for IP at 10 μg/mg lysate. FANCD2 was also immunoprecipitated by Rabbit Anti-FANCD2 Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_001018125.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:100- 1:500 |
| immunoprecipitation (IP): 5-15 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q9BXW9  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0243 maps to a region between residue 1401 and 1451 of human Fanconi anemia, complementation group D2 using the numbering given in entry NP_001018125.1 (GeneID 2177). |
| Other Notes: | FANCD2 (Fanconi anemia, complementation group D) is a protein involved in DNA repair. Defects in FANCD2 are the cause of Fanconi anemia, a heterogenous autosomal recessive disorder characterized by congenital malformations and a predisposition to cancer. The FANCD2 gene is one of 13 complementation groups that include FANCA, FANCB, FANCC, FANCD1/BRCA2, FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ/BRIP1, FANCL, FANCM, and FANCN/PALB2. The FANC members are not homologous proteins but are related by their assembly into a common nuclear complex. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |