Rabbit anti-ARID4B Antibody, Affinity Purified
SIGMA/PLA0247 - Powered by Bethyl Laboratories, Inc.
Synonym: 180 kDa Sin3-associated polypeptide; ARID domain-containing protein 4B; AT rich interactive domain 4B (RBP1- like); AT rich interactive domain 4B (RBP1-like); BCAA; BRCAA1; RBBP1L1; RBP1L1; Rb-binding protein homolog; SAP180; SIN3A-associated protein 180; breast cancer-associated antigen 1; breast cancer-associated antigen BRCAA1; breast carcinoma-associated antigen; histone deacetylase complex subunit SAP180; retinoblastoma binding protein 1-like 1; retinoblastoma-
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-ARID4B Antibody, Affinity Purified: Cat. No. PLA0247: Detection of Human ARID4B by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-ARID4B (Cat. No. PLA0247) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB staining.
Western Blotting Rabbit Anti-ARID4B Antibody, Affinity Purified: Cat. No. PLA0247: Detection of Human ARID4B by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-ARID4B Antibody PLA0247 used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 10 μg/mg lysate. ARID4B was also immunoprecipitated by a Rabbit Anti-ARID4B Antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunoprecipitation Rabbit Anti-ARID4B Antibody, Affinity Purified: Cat. No. PLA0247: Detection of Human ARID4B by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit anti-ARID4B antibody Cat. No. PLA0247 used for WB at 1 μg/mL and used for IP at 10 μg/mg lysate. ARID4B was also immunoprecipitated by Rabbit anti-ARID4B antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_057458.4 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1,000- 1:5,000 |
| immunoprecipitation (IP): 5-10 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q4LE39  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0247 maps to a region between residue 125 and 175 of human AT rich interactive domain 4B using the numbering given in entry NP_057458.4 (GeneID 51742). |
| Other Notes: | ARID4B (AT-rich interactive domain-containing protein 4B) is also known as RbBP1L1 (retinoblastoma binding protein 1-like 1) and is a retinoblastoma binding protein and member of the AT-rich interaction domain (ARID) family of proteins. In addition to the ARID DNA binding domain, ARID4B also contains a chromo domain and a TUDOR domain, indicating a function in chromatin organization and protein-protein interactions with methylated protein substrates. As a subunit of the SIN3A transcriptional co-repressor, ARID4B is implicated to be involved in chromatin remodeling and epigenetic modifications. ARID4B may play a role in the pathogenesis of breast cancer and other malignancies. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |