Rabbit anti-WDR5 Antibody, Affinity Purified
SIGMA/PLA0256 - Powered by Bethyl Laboratories, Inc.
Synonym: BIG-3; BMP2-induced 3-kb gene protein; CFAP89; SWD3; SWD3; SWD3; Set1c WD40 repeat protein; Set1c WD40 repeat protein; cilia and flagella associated protein 89; homolog; homolog (S. cerevisiae)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-WDR5 Antibody, Affinity Purified: Cat. No. PLA0256: Detection of Human WDR5 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-WDR5 (Cat. No. PLA0256) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-WDR5 Antibody, Affinity Purified: Cat. No. PLA0256: Detection of Human and Mouse WDR5 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-WDR5 Antibody PLA0256 used for WB at 0.04 μg/mL (A) and 1 μg/mL (B) and used for IP at 10 μg/mg lysate. WDR5 was successfully immunoprecipitated by a Rabbit Anti-WDR5 Antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Western Blotting Rabbit Anti-WDR5 Antibody, Affinity Purified: Cat. No. PLA0256: Detection of Mouse and Rat WDR5 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, TCMK-1, 4T1, CT26.WT, and rat C6 cells. Antibodies: Affinity purified Rabbit Anti-WDR5 Antibody PLA0256 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Rabbit anti-WDR5 Antibody, Affinity Purified: Cat. No. PLA0256: Detection of Human WDR5 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-WDR5 Antibody Cat. No. PLA0255 used for WB at 1 μg/mL and for IP at 10 μg/mg lysate. WDR5 was successfully immunoprecipitated by Rabbit Anti-WDR5 Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_060058.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse, rat |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:1,000 |
| proximity ligation assay: suitable | |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | P61964  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0256 maps to a region between residue 50 and 100 of human WD repeat domain 5 using the numbering given in entry NP_060058.1 (GeneID 11091). |
| Other Notes: | WD-repeat domain 5 (WDR5) is a member of the WD repeat family. The WD repeat is defined by four or more repeating units of a conserved core of approximately 40 amino acids ending with tryptophan-aspartic acid (WD). WD repeats may serve as sites of protein-protein interaction for adaptor proteins and facilitate multiprotein complex formation. WDR5 contains 7 WD repeats and is a core subunit of the human MLL and SET1 histone H3 lys4 methyltransferase complexes. WDR5 functions in facilitating the assembly of MLL and SET1 chromatin-modifiying complexes. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |