Rabbit anti-RuvBL2 Antibody, Affinity Purified
SIGMA/PLA0259 - Powered by Bethyl Laboratories, Inc.
Synonym: 48 kDa TATA box-binding protein-interacting protein; 48 kDa TBP-interacting protein; 51 kDa erythrocyte cytosolic protein; 51-KD; CGI-46; ECP-51; ECP51; INO80 complex subunit J; INO80J; RVB2; Reptin 52; Reptin52; RuvB (E coli homolog)-like 2; RuvB-like 2 (E. coli); TAP54-beta; TIH2; TIP48; TIP49b; TIP60-associated protein 54-beta; erythrocyte cytosolic protein; repressing pontin 52; reptin; reptin52 protein
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-RuvBL2 Antibody, Affinity Purified: Cat. No. PLA0259: Detection of Human RuvBL2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-RuvBL2 (Cat. No. PLA0259) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-RuvBL2 Antibody, Affinity Purified: Cat. No. PLA0259: Detection of Human and Mouse RuvBL2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-RuvBL2 Antibody PLA0259 used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 10 μg/mg lysate. RuvBL2 was also immunoprecipitated by a Rabbit Anti-RuvBL2 Antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunoprecipitation Rabbit Anti-RuvBL2 Antibody, Affinity Purified: Cat. No. PLA0259: Detection of Human RuvBL2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-RuvBL2 Antibody Cat. No. PLA0259 used for WB at 1 μg/mL and for IP at 10 μg/mg lysate. RuvBL2 was also immunoprecipitated by Rabbit Anti-RuvBL2 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_006657.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500- 1:2,000 |
| immunoprecipitation (IP): 5-15 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q9Y230  |
| Biochem/physiol Actions: | RuvB-like 2 (RUVBL2) plays a regulatory role in the Fanconia anemia (FA) pathway, the assembly of the telomerase holoenzyme, and nonsense-mediated mRNA decay (NMD). It may play a role in tumorigenesis and cancer progression. Overexpression of the RUVBL2 protein is observed in hepatocellular carcinoma (HCC), renal cell carcinoma, colorectal cancer, gastric cancer, breast cancer, and salivary gland cancer. It can be used as a promising target for preventing and treating HCC. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | RuvB-like 2 (RUVBL2), a transcription factor, belongs to the AAA+ protein subfamily of conserved ATPases linked with diverse cellular functions. A distinctive domain II (DII) protrudes from one side of the hexamer and defines two separate faces of the ring in RUVBL2. |
| Immunogen: | The epitope recognized by PLA0259 maps to a region between residue 1 and 50 of human RuvB-like 2 using the numbering given in entry NP_006657.1 (GeneID 10856). |
| Other Notes: | RuvB-like 2 (RuvBL2) is the second human homologue of the bacterial RuvB gene. Bacterial RuvB protein is a DNA helicase essential for homologous recombination and DNA double-strand break repair. Functional analysis showed that this protein has both ATPase and DNA helicase activities [taken from NCBI Entrez Gene (GeneID: 9400)]. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |