Rabbit anti-BRD2 Antibody, Affinity Purified
SIGMA/PLA0261 - Powered by Bethyl Laboratories, Inc.
Synonym: D6S113E; FSH; FSRG1; KIAA9001; NAT; O27.1.1; RING3; RNF3; female sterile homeotic-related gene 1; really interesting new gene 3 protein
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-BRD2 (Cat. No.PLA0261) used at a dilution of 1:200 (1 μg/mL). Detection: DAB staining.
Immunocytochemistry Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Immunocytochemistry. Sample: Formaldehyde-fixed asynchronous HeLa cells. Antibody: Affinity purified Rabbit Anti-BRD2 (Cat. No.PLA0261) used at a dilution of 1:100 (2 μg/mL). Detection: Red-fluorescent goat Anti-Rabbit IgG cross-adsorbed Antibody DyLight® 594 conjugated.
Western Blotting Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa and 293T cells. Antibodies: Affinity purified Rabbit Anti-BRD2 Antibody (Cat. No. PLA0261) used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-BRD2 Antibody, Affinity Purified: Cat. No. PLA0261: Detection of Human BRD2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified Rabbit Anti-BRD2 antibody Cat. No. PLA0261 used for IP at 6 μg per reaction. BRD2 was also immunoprecipitated by BRD2 Antibody B. For blotting immunoprecipitated BRD2, PLA0261 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_005095.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: 1:100-1:500 |
| immunohistochemistry: 1:100-1:500 | |
| immunoprecipitation (IP): 2-10 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P25440  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0261 maps to a region between residue 675 and 725 of human bromodomain containing 2 using the numbering given in entry NP_005095.1 (GeneID 6046). |
| Other Notes: | BRD2 (bromodomain-containing 2) is a nuclear mitogen-activated kinase that plays a role in transcription of cell-cycle-regulated genes by functioning as an adapter protein that recruits E2F and chromatin remodeling factors to E2F response elements. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |