Rabbit anti-TNIK Antibody, Affinity Purified
SIGMA/PLA0265 - Powered by Bethyl Laboratories, Inc.
Synonym: KIAA0551
Product Type: Product-on-demand
Western Blotting Rabbit Anti-TNIK Antibody, Affinity Purified: Cat. No. PLA0265: Detection of Human TNIK by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-TNIK Antibody PLA0265 used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 3 μg/mg lysate. TNIK was also immunoprecipitated by a Rabbit Anti-TNIK Antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunoprecipitation Rabbit Anti-TNIK Antibody, Affinity Purified: Cat. No. PLA0265: Detection of Human TNIK by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-TNIK Antibody Cat. No. PLA0265 used for WB at 1 μg/mL and used for IP at 3 μg/mg lysate. TNIK was also immunoprecipitated by Rabbit Anti-TNIK Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_055843.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q9UKE5  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0265 maps to a region between residue 600 and 650 of human TRAF2 and NCK interacting kinase using the numbering given in entry NP_055843.1 (GeneID 23043). |
| Other Notes: | TNIK (TRAF2 and NCK-interacting protein kinase) is a serine threonine kinase that acts as an essential activator of the Wnt signaling pathway. TNIK is recruited to promoters of Wnt target genes and is required to activate Wnt target genes expression. TNIK may also play a role in the response to environmental stress and appears to act upstream of the JUN N-terminal pathway [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |