Rabbit anti-ORC2 Antibody, Affinity Purified
SIGMA/PLA0267
Synonym: ORC2L; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex; origin recognition complex protein 2 homolog; subunit 2; subunit 2 (yeast homolog)-like; subunit 2 homolog; subunit 2 homolog (yeast); subunit 2-like (yeast)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-ORC2 Antibody, Affinity Purified: Cat. No. PLA0267: Detection of Human ORC2 by Immunohistochemistry. Sample: FFPE section of Human prostate carcinoma. Antibody: Affinity purified Rabbit Anti-ORC2 (Cat. No. PLA0267) used at a dilution of 1:1,000 (0.2 μg/mg) Detection: DAB staining.
Western Blotting Rabbit Anti-ORC2 Antibody, Affinity Purified: Cat. No. PLA0267: Detection of Human ORC2 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-ORC2 Antibody PLA0267 used for WB at 0.04 μg/mL (A) and 0.4 μg/mL (B) and used for IP at 3 μg/mg lysate. ORC2 was also immunoprecipitated by a Rabbit Anti-ORC2 Antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 30 seconds (A) and 10 seconds (B).
Immunoprecipitation Rabbit Anti-ORC2 Antibody, Affinity Purified: Cat. No. PLA0267: Detection of Human ORC2 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa celss (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-ORC2 Antibody Cat. No. PLA0267 used for WB at 0.4 μg/mL and for IP at 3 μg/mg lysate. ORC2 was also immunoprecipitated by Rabbit Anti-ORC2 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_006181.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:1,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q13416  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0267 maps to a region between residue 1 and 50 of human Origin Recognition Complex, Subunit 2-like using the numbering given in entry NP_006181.1 (GeneID 4999). |
| Other Notes: | The origin recognition complex (ORC) is a highly conserved six subunits protein complex essential for the initiation of the DNA replication in eukaryotic cells. Studies in yeast demonstrated that ORC binds specifically to origins of replication and serves as a platform for the assembly of additional initiation factors such as Cdc6 and Mcm proteins. ORC2 is also known as ORC2L and is a subunit of the human ORC complex. ORC2 forms a core complex with ORC3L, -4L, and -5L [taken from NCBI Entrez Gene (GeneID: 4999)] |
| Physical form: | Tris-buffered Saline containing 0.1% BSA with 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |