Rabbit anti-HSP60 Antibody, Affinity Purified
SIGMA/PLA0269 - Powered by Bethyl Laboratories, Inc.
Synonym: 60 kDa chaperonin; CPN60; GroEL; HLD4; HSP-60; HSP60; HSP65; Heat shock protein 60; HuCHA60; P60 lymphocyte protein; SPG13; chaperonin 60; heat shock 60kD protein 1 (chaperonin); heat shock 60kDa protein 1 (chaperonin); heat shock protein 65; mitochondrial matrix protein P1; short heat shock protein 60 Hsp60s1; spastic paraplegia 13 (autosomal dominant)
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-HSP60 Antibody, Affinity Purified: Cat. No. PLA0269: Detection of Human HSP60 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-HSP60 (Cat. No. PLA0269) used at a dilution of 1:200 (1 μg/mL). Detection: Vector Laboratories ImmPACT NovaRED Peroxidase Substrate
Western Blotting Rabbit Anti-HSP60 Antibody, Affinity Purified: Cat. No. PLA0269: Detection of Human and Mouse HSP60 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded), 293T (Lane T; 50 μg), and Mouse NIH3T3 (Lane M; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-HSP60 Antibody PLA0269 used for WB at 0.04 μg/mL (A) and 0.4 μg/mL (B) and used for IP at 3 μg/mg lysate. HSP60 was also immunoprecipitated by Rabbit Anti-HSP60 Antibodies which recognize other epitopes. Detection: Chemiluminescence with exposure times of 10 seconds (A and B).
Immunoprecipitation Rabbit Anti-HSP60 Antibody, Affinity Purified: Cat. No. PLA0269: Detection of Human HSP60 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-HSP60 Antibody Cat. No. PLA0269 used for WB at 0.4 μg/mL and for IP at 3 μg/mg lysate. HSP60 was also immunoprecipitated by Rabbit Anti-HSP60 Antibody B and C, which recognize other epitopes. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_002147.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:1,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Biochem/physiol Actions: | The protein encoded by the HSPD1 (heat shock protein family D member 1) gene forms a double-barrel chaperonin complex with heat shock protein 10 (HSP10). This chaperonin complex facilitates folding of proteins to their native state in the mitochondrial matrix space. Missense mutation in the HSP60 gene has been associated with a rare dominant form of hereditary spastic paraplegia and a recessively inherited white matter disorder called MitCHAP60 disease. HSP60 is also found to interact with the transcription factor interferon regulatory factor 3 (IRF3), which is involved in the induction of IFN-β (interferon- β) signaling pathway, a crucial host-protective response. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | The HSPD1 (heat shock protein family D member 1) gene encodes the HSP60 (heat shock protein 60) subunit of the HSP60/HSP10 chaperonin complex. The HSPD1 gene consisting of 12 exons is localized to human chromosome 2q33.1. |
| Immunogen: | The epitope recognized by PLA0269 maps to a region between residue 250 and 300 of human Heat Shock Protein 60 using the numbering given in entry NP_002147.2 (GeneID 3329). |
| Other Notes: | Heat shock protein 60 (HSP60) is a member of the chaperonin family. HSP60 is a mitochondrial protein that may function as a signaling molecule in the innate immune system. It is essential for the folding and assembly of newly imported proteins in the mitochondria. [taken from NCBI Entrez Gene (GeneID: 3329)]. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |