Rabbit anti-PolA1 Antibody, Affinity Purified
SIGMA/PLA0270 - Powered by Bethyl Laboratories, Inc.
Synonym: DNA polymerase alpha 1 catalytic subunit; DNA polymerase alpha catalytic subunit p180; DNA polymerase alpha p180 subunit; N syndrome (mental retardation; NSX; POLA; alpha; alpha (70kD); alpha 1; alpha 1; catalytic subunit; chromosome breakage); malformations; p180; polymerase (DNA directed); polymerase (DNA directed); polymerase (DNA directed); polymerase (DNA-directed)
Product Type: Product-on-demand
Western Blotting Rabbit Anti-PolA1 Antibody, Affinity Purified: Cat. No. PLA0270: Detection of Human PolA1 by Western Blot Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-PolA1 Antibody PLA0270 used for WB at 0.04 μg/mL (A) and 0.4 μg/mL (B) and used for IP at 3 μg/mg lysate. PolA1 was also immunoprecipitated by a Rabbit Anti-PolA1 Antibody which recognizes an upstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 30 seconds (B).
Immunoprecipitation Rabbit Anti-PolA1 Antibody, Affinity Purified: Cat. No. PLA0270: Detection of Human PolA1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-PolA1 Antibody Cat. No. PLA0270 used for WB at 0.4 μg/mL and for IP at 3 μg/mg lysate. PolA1 was also immunoprecipitated by Rabbit Anti-PolA1 Antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_058633.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-5 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P09884  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0270 maps to a region between residue 1175 and 1225 of human Polymerase (DNA directed), Alpha 1, Catalytic Subunit using the numbering given in entry NP_058633.2 (GeneID 5422). |
| Other Notes: | DNA polymerase alpha catalytic subunit (PolA1) is the catalytic subunit of the DNA polymerase alpha complex. The DNA polymerase alpha complex is composed of PolA1, PolA2, and two primase subunits, PRIM1 and PRIM2. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |