Rabbit anti-USP8 Antibody, Affinity Purified
SIGMA/PLA0272 - Powered by Bethyl Laboratories, Inc.
Synonym: HumORF8; KIAA0055; SPG59; UBPY; Ubiquitin thioesterase 8; deubiquitinating enzyme 8; hUBPy; ubiquitin isopeptidase Y; ubiquitin specific protease 8; ubiquitin thiolesterase 8; ubiquitin-
Product Type: Product-on-demand
Immunohistochemistry Rabbit Anti-USP8 Antibody, Affinity Purified: Cat. No. PLA0272: Detection of Human USP8 by Immunohistochemistry. Sample: FFPE section of Human breast carcinoma. Antibody: Affinity purified Rabbit Anti-USP8 (Cat. No. PLA0272) used at a dilution of 1:200 (1 μg/mL). Detection: DAB
Western Blotting Rabbit Anti-USP8 Antibody, Affinity Purified: Cat. No. PLA0272: Detection of Human USP8 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate from HeLa (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) and 293T (Lane T; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-USP8 Antibody PLA0272 used for WB at 0.04 μg/mL (A) and 0.4 μg/mL (B) and used for IP at 3 μg/mg lysate. USP8 was also immunoprecipitated by a Rabbit Anti-USP8 Antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Immunoprecipitation Rabbit Anti-USP8 Antibody, Affinity Purified: Cat. No. PLA0272: Detection of Human USP8 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-USP8 Antibody Cat. No. PLA0272 used for WB at 0.4 μg/mL and used for IP at 3 μg/mg lysate. USP8 was also immunoprecipitated by Rabbit Anti-USP8 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_005145.3 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:200- 1:1,000 |
| immunoprecipitation (IP): 2-5 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P40818  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0272 maps to a region between residue 400 and 450 of human Ubiquitin Specific Peptidase 8 using the numbering given in entry NP_005145.3 (GeneID 9101). |
| Other Notes: | USP8 (ubiquitin-specific processing protease 8) is also known as UBPY (ubiquitin isopeptidase Y). USP8 is a hydrolase that can remove conjugated ubiquitin from proteins and therefore plays an important regulatory role at the level of protein turnover by preventing degradation [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/ P40818]. |
| Physical form: | Tris-buffered Saline containing 0.1% BSA containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |