Rabbit anti-EPC1 Antibody, Affinity Purified
SIGMA/PLA0275 - Powered by Bethyl Laboratories, Inc.
Synonym: Epl1; enhancer of polycomb homolog 1 (Drosophila)
Product Type: Product-on-demand
Western Blotting Rabbit Anti-EPC1 Antibody, Affinity Purified: Cat. No. PLA0275: Detection of Human EPC1 by Western Blot and Immunoprecipitation. Samples: Whole cell lysate (5, 15 and 50 μg for WB; 1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-EPC1 Antibody PLA0275 used for WB at 0.1 μg/mL (A) and 1 μg/mL (B) and used for IP at 6 μg/mg lysate. EPC1 was also immunoprecipitated by a Rabbit Anti-EPC1 Antibody which recognizes a downstream epitope. Detection: Chemiluminescence with exposure times of 3 minutes (A) and 10 seconds (B).
Immunoprecipitation Rabbit Anti-EPC1 Antibody, Affinity Purified: Cat. No. PLA0275: Detection of Human EPC1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP, 20% of IP loaded) from HeLa cells. Antibodies: Affinity purified Rabbit Anti-EPC1 Antibody Cat. No. PLA0275 used for WB at 1 μg/mL and used for IP at 6 μg/mg lysate. EPC1 was also immunoprecipitated by Rabbit Anti-EPC1 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_079485.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2-10 μg/mg |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | Q9H2F5  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0275 maps to a region between residue 325 and 375 of human Enhancer of Polycomb 1 using the numbering given in entry NP_079485.1 (GeneID 80314). |
| Other Notes: | Enhancer of polycomb homolog 1 (EPC1) is a component of the NuA4 histone acetyltransferase (HAT) complex which is involved in transcriptional activation of select genes principally by acetylation of nucleosomal histones H4 and H2A. This modification may both alter nucleosome - DNA interactions and promote interaction of the modified histones with other proteins which positively regulate transcription. This complex may be required for the activation of transcriptional programs associated with oncogene and proto-oncogene mediated growth induction, tumor suppressor mediated growth arrest and replicative senescence, apoptosis, and DNA repair. NuA4 may also play a direct role in DNA repair when directly recruited to sites of DNA damage [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |