Rabbit anti-CDK1 Antibody, Affinity Purified
SIGMA/PLA0287 - Powered by Bethyl Laboratories, Inc.
Synonym: CDC2; CDC28A; CDK1; G1 to S and G2 to M; P34CDC2; cell cycle controller CDC2; cell division control protein 2 homolog; cell division cycle 2; cell division protein kinase 1; p34 protein kinase
Product Type: Product-on-demand
Immunoprecipitation Rabbit Anti-CDK1 Antibody, Affinity Purified: Cat. No. PLA0287: Detection of Human CDK1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells 1 mg for IP, 20% of IP loaded), 293T (T; 50 μg) and Jurkat (J; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-CDK1 antibody Cat. No. PLA0287 used for WB at 0.4 μg/mL and for IP at 6 μg/mg lysate. CDK1 was also immunoprecipitated by Rabbit Anti-CDK1 Antibody B, which recognizes a downstream epitope. Detection: Chemiluminescence with exposure time of 30 seconds.
| accession no. | NP_001777.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 2- 10 μg/mg |
| western blot: 1:2,000-1:10,000 | |
| UniProt accession no. | P06493  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0287 maps to a region between residue 1 and 50 of human Cyclin-Dependent Kinase 1 using the numbering given in entry NP_001777.1 (GeneID 983). |
| Other Notes: | Cyclin-dependent kinase 1 (CDK1) is a member of the Ser/Thr protein kinase family. CDK1 is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of CKD1 is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control [taken from NCBI Entrez Gene (Gene ID: 983)]. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |