Rabbit anti-Coilin Antibody, Affinity Purified
SIGMA/PLA0290 - Powered by Bethyl Laboratories, Inc.
Synonym: CLN80; coilin p80; p80; p80-coilin
Product Type: Product-on-demand
Western Blotting Rabbit Anti-Coilin Antibody, Affinity Purified: Cat. No. PLA0290: Detection of Human Coilin by Western Blot. Samples: Whole cell lysate from HeLa (15 and 50 μg), 293T (T; 50 μg) and Jurkat (J; 50 μg) cells. Antibodies: Affinity purified Rabbit Anti-Coilin antibody Cat. No. PLA0290 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with exposure time of 30 seconds.
Immunoprecipitation Rabbit Anti-Coilin Antibody, Affinity Purified: Cat. No. PLA0290: Detection of Human Coilin by Western Blot of Immunoprecipitates. Samples: Whole cell lysate from HeLa cells (1 mg for IP, 20% of IP loaded). Antibodies: Affinity purified Rabbit Anti-Coilin antibody Cat. No. PLA0290 used for WB at 1 μg/mL and for IP at 6 μg/mg lysate. Coilin was also immunoprecipitated by Rabbit Anti-Coilin antibody B, which recognizes an upstream epitope. Detection: Chemiluminescence with exposure time of 10 seconds.
| accession no. | NP_004636.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500-1:2,000 |
| immunoprecipitation (IP): 2- 10 μg/mg | |
| western blot: 1:2,000- 1:10,000 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0290 maps to a region between residue 526 and 576 of human Coilin using the numbering given in entry NP_004636.1 (GeneID 8161). |
| Other Notes: | Coilin is a component of the nuclear coiled bodies (CBS) which are involved in the function or assembly/disassembly of nucleoplasmic snRNPs. During mitosis, CBS disassemble, coinciding with a mitotic-specific phosphorylation of p80 coilin [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |