Goat anti-CRSP1/TRAP220 Antibody, Affinity Purified
SIGMA/PLA0300 - Powered by Bethyl Laboratories, Inc.
Synonym: ARC205; CRSP1; CRSP200; DRIP205; DRIP230; Mediator complex subunit 1; PBP; PPAR binding protein; PPAR-binding protein; PPARBP; PPARG binding protein; PPARGBP; RB18A; TR-interacting protein 2; TRAP220; TRIP-2; TRIP2; activator-recruited cofactor 205 kDa component; p53 regulatory protein RB18A; peroxisome proliferator-
Product Type: Product-on-demand
Western Blotting Goat Anti-CRSP1/TRAP220 Antibody, Affinity Purified: Cat. No. PLA0300: Detection of Human and Mouse CRSP1/TRAP220 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), Jurkat (50 μg), and Mouse NIH3T3 (50 μg) cells. Antibodies: Affinity purified goat Anti-CRSP1/TRAP220 Antibody PLA0300 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Goat Anti-CRSP1/TRAP220 Antibody, Affinity Purified: Cat. No. PLA0300: Detection of Human CRSP1/TRAP220 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-CRSP1/TRAP220 antibody Cat. No. PLA0300 used for IP at 6 μg/mg lysate. CRSP1/TRAP220 was also immunoprecipitated by Rabbit Anti-CRSP1/TRAP220 antibody Cat. No. PLA0142. For blotting immunoprecipitated CRSP1/TRAP220, PLA0142 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
| accession no. | NP_004765.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human, mouse |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:500-1:2,000 |
| immunoprecipitation (IP): 2- 10 μg/mg | |
| western blot: 1:500- 1:2,500 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0300 maps to a region between residue 1531 and 1581 of human Cofactor Required for Sp1 Transcriptional Activation 1/Thyroid Hormone Receptor-Associated Protein Complex Component using the numbering given in entry NP_004765.2 (GeneID 5469). |
| Other Notes: | CSP1/TRAP220 is a component of the Mediator complex, a coactivator involved in the regulated transcription of nearly all RNA polymerase II-dependent genes. Mediator functions as a bridge to convey information from gene-specific regulatory proteins to the basal RNA polymerase II transcription machinery. Mediator is recruited to promoters by direct interactions with regulatory proteins and serves as a scaffold for the assembly of a functional preinitiation complex with RNA polymerase II and the general transcription factors [taken from the Universal Protein Resource (UniProt) www.uniprot.org/uniprot/Q |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |