Goat anti-Sp1 Antibody, Affinity Purified
SIGMA/PLA0307 - Powered by Bethyl Laboratories, Inc.
Synonym: specificity protein 1
Product Type: Product-on-demand
Immunohistochemistry Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Mouse Sp1 by Immunohistochemistry. Sample: FFPE section of Mouse renal cell carcinoma. Antibody: Affinity purified Rabbit Anti-Sp1 (Cat. No. PLA0307) used at a dilution of 1:1,000 (1 μg/mL). Detection: DAB
Immunohistochemistry Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Human Sp1 by Immunohistochemistry. Sample: FFPE section of Human ovarian carcinoma. Antibody: Affinity purified Rabbit Anti-Sp1 (Cat. No. PLA0307) used at a dilution of 1:5,000 (0.2 μg/mL). Detection: DAB
Western Blotting Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Human Sp1 by Western Blot. Samples: Whole cell lysate from 293T (15 and 50 μg), HeLa (50 μg), and Jurkat (50 μg) cells. Antibodies: Affinity purified goat Anti-Sp1 Antibody PLA0307 used for WB at 0.1 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blotting Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Mouse Sp1 by Western Blot. Samples: Whole cell lysate (50 μg) from NIH3T3, Renca, and TCMK-1 cells. Antibodies: Affinity purified goat Anti-Sp1 Antibody PLA0307 used for WB at 0.4 μg/mL. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation Goat Anti-Sp1 Antibody, Affinity Purified: Cat. No. PLA0307: Detection of Human Sp1 by Western Blot of Immunoprecipitates. Samples: Whole cell lysate (1 mg for IP; 20% of IP loaded) from 293T cells. Antibodies: Affinity purified goat Anti-Sp1 antibody Cat. No. PLA0307 used for IP at 6 μg/mg lysate. Sp1 was also immunoprecipitated by Rabbit Anti-Sp1 antibody Cat. No. PLA0044. For blotting immunoprecipitated Sp1, PLA0307 was used at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 30 seconds.
| accession no. | NP_612482.2 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | goat |
| clone | polyclonal |
| concentration | 1 mg/mL |
| conjugate | unconjugated |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | mouse, human |
| storage temp. | 2-8°C |
| target post-translational modification | unmodified |
| technique(s) | immunohistochemistry: 1:1,000-1:5,000 |
| immunoprecipitation (IP): 2- 10 μg/mg | |
| western blot: 1:2,000- 1:10,000 | |
| UniProt accession no. | P08047  |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | The epitope recognized by PLA0307 maps to a region between residue 735 to 785 of human Sp1 Transcription Factor using the numbering given in entry NP_612482.2 (GeneID 6667). |
| Other Notes: | Specificity protein 1 (Sp1) is the original member of the Sp1-like/KLF family of zinc-finger transcription factors that binds GC-rich DNA elements to regulate transcription of genes involved in a variety of cellular functions. Sp-1 interacts with coactivators such as p300/CBP, TAFII130, and CRSP. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |