Rabbit anti-Phospho RPA32 (S33) Antibody, Affinity Purified
SIGMA/PLA0310
Synonym: 32kDa; REPA2; RF-A protein 2; RP-A p32; RP-A p34; RPA32; replication factor A protein 2; replication protein A 34 kDa subunit; replication protein A2; replication protein A2 (32kD)
Product Type: Product-on-demand
Western Blotting Rabbit Anti-Phospho RPA32 (S33) Antibody, Affinity Purified: Cat. No. PLA0310: Detection of Human phospho RPA32-S33 by Western Blot. Samples: Whole cell lysate (50 μg) from HeLa cells that were either treated with 100 μM etoposide for 2 hours (+) or mock treated (-). Antibodies: Affinity purified Rabbit Anti-phospho RPA32-S33 Antibody PLA0310 used for WB at 1 μg/mL. Detection: Chemiluminescence with an exposure time of 10 seconds. Lower panel shows Westen Blotting for total RPA32 using affinity purified Rabbit Anti-RPA32 Antibody PLA0070 at 0.1 μg/mL with an exposure time of 10 seconds.
| accession no. | NP_002937.1 |
| antibody form | affinity purified immunoglobulin |
| antibody product type | primary antibodies |
| biological source | rabbit |
| Quality Level | 100  |
| shipped in | wet ice |
| species reactivity | human |
| storage temp. | 2-8°C |
| target post-translational modification | phosphorylation (pSer33) |
| technique(s) | western blot: 1:1,000-1:5,000 |
| Biochem/physiol Actions: | Replication protein A2 (RPA2) is involved in DNA repair and replication. The protein has been shown to be phosphorylated as a response to DNA damage and apoptosis in vitro. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Replication protein A2 (RPA2) is a 32kDa protein and one of the subunits of replication protein A complex. The gene encoding it is localized on human chromosome 1p35. |
| Immunogen: | Immunogen was a phosphorylated synthetic peptide, which represented a portion of human replication protein A2, 32 kDa surrounding phosphorylated serine that corresponded to position 33 using the numbering given in entry NP_002937.1 (GeneID 6118). |
| Other Notes: | Replication protein A (RPA) is a multisubunit complex that carries out DNA mismatch repair (MMR) in association with MSH2 (a subunit of human MutS heterodimers), MLH1 (a subunit of human MutL heterodimers), PCNA, and DNA polymerase-delta. RPA is composed of a heterotrimer that includes subunits of 70 kDa (RPA1), 32kDa (RPA2), and 14kDa (RPA3). RPA2, the 32kDa subunit, is phosphorylated by the cdc2 family of kinases when cells enter S-phase and in response to DNA damage by ATM, ATR, and DNA-PK. |
| Physical form: | Tris-citrate/phosphate buffer, pH 7 to 8 containing 0.09% Sodium Azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | nwg |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352203 |