GenElute™ Endotoxin-free Plasmid Midiprep Kit
SIGMA/PLED35 - sufficient for 35 purifications
Synonym: GenElute™ Endotoxin-free Plasmid Kits; endo free midiprep plasmid isolation kit; endotoxin free plasmid extraction kit; endotoxin free plasmid isolation kit; endotoxin free plasmid purification kit; midiprep plasmid isolation kit; plasmid extraction kit; plasmid purification kit; Gen Elute
Product Type: Chemical
Comparison of time required per isolation using different Endotoxin-free plasmid isolation systems. Plasmid DNA was isolated following each manufacturers recommended protocol. Each system was tested multiple times and the average preparation time is shown here.
Comparison of transfection efficiencies of plasmids prepared with different Endotoxin-free isolation methods.
The data shows the average of six replicates for each named isolation method. All transfections were in CHO-K1 cells. The OD measurements were taken at 420 nm and the units of β-gal/plate were found using our β-Galactosidase Reporter Gene Activity Detection Kit. The cells were grown to ~60-75% confluency and transfected using 3 μg of plasmid DNA/15 μg of Escort IV. β-Gal activity was determined ~60-70 hour post-transfection. Error bars are the standard deviation of the 6 replicate wells tested.
The data shows the average of six replicates for each named isolation method. All transfections were in CHO-K1 cells. The OD measurements were taken at 420 nm and the units of β-gal/plate were found using our β-Galactosidase Reporter Gene Activity Detection Kit. The cells were grown to ~60-75% confluency and transfected using 3 μg of plasmid DNA/15 μg of Escort IV. β-Gal activity was determined ~60-70 hour post-transfection. Error bars are the standard deviation of the 6 replicate wells tested.
12 Principles of Green Chemistry: Principle 12—Inherently Safer Chemistry for Accident Prevention. This product utilizes safer chemistry to minimize the potential for chemical accidents, including releases, explosions and fires.
| greener alternative category | Aligned  , |
| greener alternative product characteristics | Inherently Safer Chemistry for Accident Prevention Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 35 purifications |
| Application: | The kit has been used for purifying plasmid DNA, which has been used for transfection. |
| Features and Benefits: | • High transfection efficiency and yield • Purify up to 250 μg endotoxin-free plasmid DNA (≤0.1 EU/μg DNA) • Fast and simple allowing up to 12 preps in less than 2 hours |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute Endotoxin-free Plasmid Kit offers a simple, rapid, cost effective method for purifying plasmid DNA with ≤0.1 EU/μg DNA for high efficiency transfection. Endotoxins (also known as lipopolysaccharides or LPS) are often co-purified with plasmid DNA and significantly reduce transfection efficiencies in endotoxin-sensitive cell lines. Recombinant E. coli is harvested from an overnight culture by centrifugation and subjected to a modified alkaline-SDS lysis procedure to produce a cleared lysate. Endotoxins are removed from the cleared lysate with simple extraction-phase separation steps. Plasmid DNA is further purified by adsorption onto silica in the presence of high salts. After a simple spin-wash step, the bound plasmid DNA is eluted in endotoxin-free water. Up to 1.2 mg of endotoxin-free plasmid DNA may be prepared from 25-200 mL of culture. The recovered plasmid DNA is predominately in its supercoiled form. The DNA is ready for immediate use in downstream applications such as transfection, restriction digestion, cloning, sequencing, and PCR amplification. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |    GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H290 - H302 + H332 - H315 - H319 - H334 - H336 |
| Precautionary statements | P234 - P261 - P264 - P301 + P312 - P302 + P352 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 22-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |
| Components | Resuspension Solution, Add RNase A before use. After addition of RNAse, store at 2-8ºC. ); Lysis Solution; Endotoxin Removal solution, Remove lipopolysaccharides (LPS) from plasmid DNA preparations; E-Toxate™ Water, endotoxin, free; RNase A solution; Column Preparation Solution |