GenElute™ Plasmid Miniprep Kit
SIGMA/PLN70 - sufficient for 70 purifications
Synonym: GenElute™ Plasmid Kit; plasmid extraction kit; plasmid isolation kit; plasmid purification kit; Gen Elute
Product Type: Chemical
Electropherogram revealing >600 bases of sequence from pUC-TMV using GenElute Plasmid DNA Purification Miniprep Kit.
Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI BigDye™ terminator chemistry. Sequencing reactions were resolved on an ABI Prism 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI BigDye™ terminator chemistry. Sequencing reactions were resolved on an ABI Prism 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
Electropherogram revealing sequence from pUC-TMV using GenElute™ Plasmid DNA Purification Miniprep Kit.
Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI BigDye® terminator chemistry. Sequencing reactions were resolved on an ABI Prism® 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE™ Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
Cycle Sequencing was performed using 500 ng of template, a T7 sequencing primer, and ABI BigDye® terminator chemistry. Sequencing reactions were resolved on an ABI Prism® 377 XL instrument with a 48 cm gel cassette containing 4.5% AutoPAGE™ Plus acrylamide at 2.4 kV, 48 °C for 7 hours.
12 Principles of Green Chemistry: Principle 4—Designing Safer Chemicals
Chemical products should be designed to affect their desired function while minimizing their toxicity.
Chemical products should be designed to affect their desired function while minimizing their toxicity.
| greener alternative category |  , Aligned |
| greener alternative product characteristics | Designing Safer Chemicals Learn more about the Principles of Green Chemistry . |
| Quality Level | 200 |
| storage temp. | 15-25°C |
| sustainability | Greener Alternative Product |
| technique(s) | DNA purification: suitable |
| usage | sufficient for 70 purifications |
| Application: | The DNA is ready for immediate use in applications such as restriction enzyme digestion, cloning, PCR, transformation, transcription, conventional, and automated sequencing. |
| Features and Benefits: | • Purified plasmid DNA in less than 30 minutes • Purify up to 15 μg of plasmid DNA • No detectable genomic DNA or RNA contamination • No phenol/chloroform extraction or alcohol precipitation required |
| General description: | Click here to view the kit protocol |
| General description: | The GenElute Plasmid Miniprep Kit offers a simple, rapid, and cost-effective method for isolating plasmid DNA from recombinant E. coli cultures. By combining silica-binding technology and the convenience of a spin column format, up to 15 μg of high copy plasmid DNA can be recovered from 1-5 mL of E. coli culture in less than 30 minutes. Note that actual yield and optimal volume of culture to use depend on the plasmid and the culture medium. An overnight recombinant E. coli culture is harvested with centrifugation and subjected to a modified alkaline-SDS lysis procedure followed by adsorption of the DNA onto silica in the presence of high salts. Contaminants are then removed by a spin-wash step. Finally, the bound DNA is eluted in water or Tris-EDTA buffer. |
| General description: | We are committed to bringing you Greener Alternative Products, which adhere to one or more of The 12 Principles of Greener Chemistry. This product has Inherently Safer Chemistry, compared to the standard use of phenol and chloroform to perform DNA extractions. |
| Legal Information: | GenElute is a trademark of Sigma-Aldrich Co. LLC |
| Other Notes: | For additional information, please see www.sigma-aldrich.com/gen. |
| Packaging: | Components included:• Qty in kit:2 Part number: T5449 Component description: Collection Tubes, 2 mL • Qty in kit:1 Part number: W4011 Component description: Optional Wash Solution • Qty in kit:1 Part number: L1912 Component description: Lysis Solution • Qty in kit:1 Part number: E5650 Component description: Elution Solution • Qty in kit:1 Part number: N5158 Component description: Neutralization/Binding Buffer • Qty in kit:1 Part number: G6415 Component description: GenElute™ plasmid mini spin column • Qty in kit:1 Part number: C2112 Component description: Column Preparation Solution • Qty in kit:1 Part number: W3886 Component description: Wash Solution Concentrate • Qty in kit:1 Part number: R1149 Component description: Resuspension Solution • Qty in kit:1 Part number: R6148 Component description: RNase A solution |
| Symbol |     GHS02,GHS05,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H226 - H290 - H302 - H315 - H319 - H334 - H336 |
| Precautionary statements | P210 - P233 - P234 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | Xn |
| Risk Statements | 10-22-36/38-67 |
| Safety Statements | 26 |
| RIDADR | UN 3316 9 |
| WGK Germany | WGK 2 |
| Storage Temp. | 15-25°C |
| UNSPSC | 41105501 |