Trypsin Profile IGD Kit
SIGMA/PP0100
Synonym: In gel digestion kit; Proteomics grade Trypsin; TPCK treated
Product Type: Chemical
Trypsin Profile IGD Kit: Cat. No. PP0100
| Quality Level | 200  |
| storage temp. | 2-8°C |
| usage | kit sufficient for ≤100 applications |
| Application: | Trypsin Profile IGD Kit has been used for in-gel digestion for LC-MS (liquid chromatography-mass spectrometry)/MS and MALDI-TOF (matrix assisted laser desorption ionization-time of flight) mass spectrometry analysis. |
| Features and Benefits: | • Faster destaining than with alternative in-gel digest kits • Includes 100% of the reagents needed to destain, digest, and extract proteins/peptides of interest • Compatible with Coomassie, SYPRO™ Orange, SYPRO Ruby, and ProteoSilver™ Stained gels • Resulting samples are ready for analysis by MALDI-MS or HPLC-MS |
| General description: | The Trypsin Profile IGD Kit enables fast, efficient, and complete in-gel tryptic digestion of up to 100 excised protein spots. Digested proteins are ready for MALDI-MS (matrix-assisted laser desorption/ionization-mas Trypsin has been chemically modified through reductive methylation of the ε-amino groups of lysine to reduce autolysis and minimize autolytic fragments. In addition, it has been TPCK (tosyl phenylalanyl chloromethyl ketone) treated to remove residual chymotrypsin activity and then further purified by affinity chromatography, yielding a highly purified trypsin suitable for proteomics work. |
| Legal Information: | ProteoSilver is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | SYPRO is a trademark of Thermo-Fisher Scientific, Inc. |
| Symbol |    GHS02,GHS07,GHS08 |
| Signal word | Danger |
| Hazard statements | H225 - H302 + H312 + H332 - H315 - H319 - H334 - H335 |
| Precautionary statements | P210 - P233 - P280 - P301 + P312 - P303 + P361 + P353 - P305 + P351 + P338 |
| Hazard Codes | F,Xn |
| Risk Statements | 11-20/21/22-36/37/38-42 |
| Safety Statements | 16-23-26-36/37-45 |
| RIDADR | UN 3316 9 |
| Flash Point(F) | 35.6 °F - closed cup |
| Flash Point(C) | 2.0 °C - closed cup |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | Destaining Solution Reconstituted to 75 mL |