GlycoProfile™ I, In-Gel Deglycosylation Kit
SIGMA/PP0200
EC Number: 232-615-7
Product Type: Chemical
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| Quality Level | 200  |
| shelf life | ≥1 yr at 2‑8 °C |
| storage temp. | 2-8°C |
| Application: | The GlycoProfile™ Enzymatic In-gel N-Deglycosylation Kit is optimized to provide a convenient, reproducible method to N-deglycosylate and digest protein samples from one dimensional (1D) and two dimensional (2D) polyacrylamide gel pieces for subsequent mass spectrometry or HPLC analysis. This procedure is suitable for Coomassie® Brilliant Blue and colloidal Coomassie stained gels. Silver stained gels may also be used if properly destained. |
| Biochem/physiol Actions: | The kit includes Proteomics Grade PNGase F and trypsin enzymes necessary for N-linked deglycosylation and tryptic digestion, respectively. The samples can then be desalted and concentrated for analysis by MALDI-TOF MS or electrospray MS with subsequent database searching. |
| Legal Information: | GlycoProfile is a trademark of Sigma-Aldrich Co. LLC |
| Preparation Note: | Each kit contains reagents sufficient to deglycosylate and digest up to 10 samples. |
| Symbol |  GHS08 |
| Signal word | Danger |
| Hazard statements | H334 |
| Precautionary statements | P261 - P284 - P501 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352200 |
| Components | PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U; Trypsin from porcine pancreas, Proteomics Grade, BioReagent, Dimethylated 20 μg; Trypsin Solubilization Reagent; Invertase Glycoprotein Standard, BioReagent, from Saccharomyces cerevisiae; Acetonitrile, ≥99.93%, BioSyn® 50 mL |