Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit
SIGMA/PP0201 - sufficient for 20 samples
Product Type: Chemical
Photo is meant to be representative of packaging you will receive if ordered. Appropriate product information is printed on actual labels and package types are subject to change
| Quality Level | 200  |
| storage temp. | 2-8°C |
| Application: | GlycoProfile II Enzymatic In-Solution N-Deglycosylation Kit was used to study the effects of glycosylation of the N-acetyl-D-galactosamine (GalNAc)-specific lectin found in Schizophyllum commune. |
| Application: | GlycoProfile™ II has been optimized to provide a convenient and reproducible method to remove N-linked glycans from glycoproteins and is compatible with subsequent MALDI-TOF mass spectrometric analysis without interference from any of the reaction components. |
| Application: | Glycoprofile™ II, Enzymatic In-Solution N-Deglycosylation Kit has been used in the deglycosylation of Schizophyllum commune lectin, Arabidopsis mannanase, human umbilical vein endothelial cells (HUVEC) protein lysate and organic solute transporter protein, Ostβ. |
| General description: | Contains sufficient reagents for a minimum of 20 reactions when the sample size is between one to two mg of a typical glycoprotein. |
| Legal Information: | GlycoProfile is a trademark of Sigma-Aldrich Co. LLC |
| Symbol |     GHS05,GHS06,GHS08,GHS09 |
| Signal word | Danger |
| Hazard statements | H301 + H331 - H310 - H315 - H317 - H318 - H334 - H361d - H373 - H410 |
| Precautionary statements | P273 - P280 - P301 + P310 - P302 + P352 + P310 - P304 + P340 + P311 - P305 + P351 + P338 |
| Hazard Codes | T,N |
| Risk Statements | 23/24/25-38-41-43-48/22-50/53 |
| Safety Statements | 26-36/37/39-45-60-61 |
| RIDADR | UN 3316 9 |
| Storage Temp. | 2-8°C |
| UNSPSC | 12352202 |
| Components | PNGase F from Elizabethkingia meningoseptica, BioReagent, ≥95% (SDS-PAGE) 50 U; Ribonuclease B from bovine pancreas, BioReagent, ≥50 Kunitz units/mg protein, ≥80% (SDS-PAGE) .5 mg; 10× Reaction Buffer 1 mL/vial; Octyl β-D-glucopyranoside, BioXtra, ≥98% (GC) 100 mg; 2-Mercaptoethanol, Molecular Biology, suitable for electrophoresis, suitable for cell culture, BioReagent, 99% (GC/titration) .9 mL |