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GlycoProfile 2-AB Labeling Kit

SIGMA/PP0520

Synonym: 2-AB Labeling Kit

Product Type: Chemical

Catalog Number PKG Qty. Price Quantity
45-PP0520-1KT 1 kit
$650.00
1/EA
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Quality Level 200 
Application: GlycoProfile 2-AB Labeling Kit has been used for the labelling of N-linked glycans from human IgG, Bovine fetuin, and human epididymis protein 4. It has also been used for labelling free oligosaccharides from mouse spleen.
Application: While 2-AB is slightly less sensitive than 2-AA, it is suitable for downstream glycan analysis by HPAE, HPLC and ESI-MS. Although suitable for the same applications, 2-AA is better suited for SDS-PAGE.
General description: The GlycoProfile labeling kits are designed for efficient labeling of N-linked, O-linked, and glycosyl­phosphatidyl­inositol (GPI) anchored glycans with either 2-aminobenzamide (2-AB) or 2-aminobenzoic acid (anthranilic acid; 2-AA). These small fluorophores increase the spectral absorption of glycans, improving the detection of labeled glycans by HPAE (high-performance anion exchange chromatography), HPLC, and ESI-MS (electrospray mass spectroscopy) methods. Binding is robust and the resulting dye-glycan conjugates are stable, with no degradation during post-labeling analysis.
Legal Information: GlycoProfile is a trademark of Sigma-Aldrich Co. LLC
Legal Information: Labeling of glycans with 2-AB is covered under US Patent No. 5,747,347 and its foreign equivalents.
UNSPSC 12352200
Components 2-AB Labeling Reagent 2 x 5 mg; Dimethyl sulfoxide (DMSO) 2 x 350 μL; Acetic acid, glacial 2 x 200 μL; Reductant (sodium cyanoborohydride) 2 x 6 mg

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