Anti-Nephrin antibody produced in rabbit
SIGMA/PRS2265 - affinity isolated antibody, buffered aqueous solution
Synonym: CNF; NPHN; Renal glomerulus-specific cell adhesion receptor
Product Type: Chemical
Immunohistochemistry Anti-Nephrin: Cat. No. PRS2265 Immunohistochemistry of Nephrin in mouse kidney tissue with Nephrin antibody at 1 μg/mL.
Immunofluorescence Immunofluorescence of Nephrin in Mouse Kidney cells with Nephrin antibody at 10 μg/mL.
Immunoblotting Anti-Nephrin: Cat. No. PRS2265 Western blot analysis of Nephrin in mouse kidney tissue lysate with Nephrin antibody at 1 μg/mL in the (A) absence and (B) presence of blocking peptide.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| NCBI accession no. | NP_004637   |
| Quality Level | 100 |
| shipped in | dry ice |
| species reactivity | rat, human, mouse |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | O60500 |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Nephrin is strongly expressed in renal glomeruli and is a member of the immunoglobulin family of cell adhesion molecules. It is a 1241-residue transmembrane protein. Mutations in the Nephrin gene result in congenital nephrotic syndrome, an autosomalrecessive disorder characterized by massive proteinuria in utero and nephrosis at birth. Renal glomeruli allow normal kidneys to filter plasma so that it is very pure. Nephrin is expressed in the podocyte slit-diaphragm of the renal glomeruli in a manner that suggests that Nephrin molecules homodimerize in an anti-parallel fashion similar to cadherin interactions in adherens junctions. Thus, Nephrin may constitute the entire extracellular structure of the slit-diaphragm. |
| Immunogen: | Nephrin antibody was raised against a 14 amino acid synthetic peptide from near the carboxy terminus of human Nephrin. The immunogen is located within the last 50 amino acids of Nephrin. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP2265 . |
| Physical form: | Solution in phosphate buffered saline containing 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |