Anti-Caspase-12 (large) antibody produced in rabbit
SIGMA/PRS3195 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Caspase 12
MDL Number: MFCD03454855
Product Type: Chemical
Immunohistochemistry Anti-Caspase-12 (Large): Cat. No. PRS3195 Immunohistochemical staining of human heart tissue using Caspase-12 antibody (large) at 2 μg/mL.
Immunohistochemistry Staining of formalin-fixed, paraffin-embedded rat brain sections with 0.05 μg/mL Anti-Caspase-12 (large) antibody produced in rabbit (Cat. No. PRS3195). The antibody was developed using goat-Anti-Rabbit IgG, DyLight 594 conjugate. (The picture is courtesy of Fabrice GANKAM MD, Ph.D., Néphrologie Dialyse et Transplantation, Hopital Erasme - ULB)
Immunofluorescence Immunofluorescence of Caspase-12 in Human Heart tissue with Caspase-12 antibody at 10 μg/mL.
Immunoblotting Anti-Caspase-12 (Large): Cat. No. PRS3195 Western blot analysis of caspase-12 in human heart lysate with caspase-12 antibody (large) at 0.5 (Lane A), 1 (Lane B), and 2 μg/mL (Lane C), respectively.
Immunoblotting Rat brain extracts were separated on TruPAGE™ precast gradient gel and probed with Anti-Caspase-12 (large) antibody produced in rabbit (Cat. No. PRS3195). The antibody was developed using a chemiluminescent substrate. Lanes 1. 0.5 μg/mL antibody (The picture is courtesy of Fabrice GANKAM MD, Ph.D., Néphrologie Dialyse et Transplantation, Hopital Erasme - ULB).
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | predicted mol wt 46 kDa |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human, mouse, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | O08736  |
| Application: | Applications in which this antibody has been used successfully, and the associated peer-reviewed papers, are given below. Western Blotting (1 paper) |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| Immunogen: | a synthetic peptide corresponding to amino acids within the large cleavage product of murine caspase-12. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3195 . |
| Physical form: | Solution in phosphate buffered saline containing 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |