Anti-Caspase-1 (ab1) antibody produced in rabbit
SIGMA/PRS3459 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Caspase 1, apoptosis-related cysteine peptidase (Interleukin 1, beta, convertase); Anti-ICE; Anti-IL-1β converting enzyme
MDL Number: MFCD00283496
Product Type: Chemical
Immunohistochemistry Anti-Caspase-1: Cat. No. PRS3459 Immunohistochemical staining of human heart tissue using Caspase-1 antibody at 2 μg/mL.
Immunoblotting Anti-Caspase-1: Cat. No. PRS3459 Western blot analysis of Caspase-1 in human heart cell lysate with Caspase-1 antibody at (A) 0.5, (B) 1, and (C) 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P29466  |
| Application: | Anti-Caspase-1 (ab1) antibody produced in rabbit has been used in western blotting and immunofluorescence staining. |
| Biochem/physiol Actions: | Caspase-1 can transform the pro-inflammatory cytokines, interleukin-1β (IL-1β) and IL-18 into their active forms. It participates in pyroptosis. It is overexpressed in the aorta of coronary atherosclerosis patients. It helps in host cell survival by inducing membrane biogenesis to restore the impairment caused by pore-forming toxins. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Caspase-1 is also known as interleukin-1β converting enzyme (ICE). It is located on chromosome 11q22.2. It has 10 exons, an N terminal CARD (caspase activation and recruitment domain), a large P20 subunit and a small P10 subunit. It is present in leukocytes, monocytes and epithelial cells. |
| Immunogen: | a 16 amino acid peptide from near the carboxy-terminus of human Caspase-1. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3459 . |
| Physical form: | Solution in phosphate buffered saline containing 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |