Anti-Caspase-1 (ab2) antibody produced in rabbit
SIGMA/PRS3463 - affinity isolated antibody, buffered aqueous solution
Synonym: Anti-Caspase 1, apoptosis-related cysteine peptidase (Interleukin 1, beta, convertase); Anti-ICE; Anti-IL-1β converting enzyme
Product Type: Chemical
Immunohistochemistry Anti-Caspase-1: Cat. No. PRS3463 Immunohistochemistry of caspase-1 in human spleen tissue with Caspase-1 antibody at 10 μg/mL.
Immunofluorescence Immunofluorescence of Caspase-1 in HeLa cells with Caspase-1 antibody at 20 μg/mL.
Immunoblotting Anti-Caspase-1: Cat. No. PRS3463 Western blot analysis of Caspase-1 in HeLa cell lysate with Caspase-1 antibody (IN) at (A) 0.5, (B) 1, and (C) 2 μg/mL.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| Quality Level | 100  |
| shipped in | dry ice |
| species reactivity | human |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunofluorescence: suitable |
| immunohistochemistry: suitable | |
| indirect ELISA: suitable | |
| western blot: suitable | |
| UniProt accession no. | P29466  |
| Biochem/physiol Actions: | Caspase-1 can transform the pro-inflammatory cytokines, interleukin-1β (IL-1β) and IL-18 into their active forms. It participates in pyroptosis. It is overexpressed in the aorta of coronary atherosclerosis patients.It helps in host cell survival by inducing membrane biogenesis to restore the impairment caused by pore-forming toxins. |
| General description: | Caspase-1 is also known as interleukin-1βconverting enzyme (ICE). It is located on chromosome 11q22.2. It has 10 exons, an N terminal CARD (caspase activation and recruitment domain), a large P20 subunit and a small P10 subunit. It is present in leukocytes, monocytes and epithelial cells. |
| Immunogen: | a 15 amino acid peptide from near the middle of human Caspase-1. |
| Other Notes: | The action of this antibody can be blocked using blocking peptide SBP3463 . |
| Physical form: | Solution in phosphate buffered saline containing 0.02% sodium azide |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |