Quantitative RT-PCR ReadyMix™
SIGMA/QR0200 - One step RT-qPCR for probe-based methods, MMLV & hot-start Taq
Synonym: 1-step RT-qPCR mix; Quantitative real-time PCR master mix
Product Type: Chemical
Representative packaging
| color | colorless |
| compatibility | ABI 5700 |
| ABI 7000 | |
| ABI 7300 | |
| ABI 7500 | |
| ABI 7500 Fast | |
| ABI 7700 | |
| ABI 7900 | |
| ABI 7900 HT | |
| ABI 7900 HT Fast | |
| ABI StepOne | |
| ABI StepOnePlus | |
| ABI ViiA 7 | |
| Bio-Rad CFX384 | |
| Bio-Rad CFX96 | |
| Bio-Rad iCycler iQ | |
| Bio-Rad iQ5 | |
| Bio-Rad MiniOpticon | |
| Bio-Rad MJ Chromo4 | |
| Bio-Rad MJ Opticon | |
| Bio-Rad MJ Opticon 2 | |
| Bio-Rad MyiQ | |
| Cepheid SmartCycler | |
| Eppendorf® Mastercycler ep realplex | |
| Eppendorf® Mastercycler ep realplex2 s | |
| Illumina Eco qPCR | |
| Qiagen Corbett Rotor-Gene 3000 | |
| Qiagen Corbett Rotor-Gene 6000 | |
| Qiagen Corbett Rotor-Gene Q | |
| Roche LightCycler 480 | |
| Strategene Mx3000P | |
| Strategene Mx3005P | |
| Strategene Mx4000 | |
| detection method | probe-based |
| feature | dNTPs included |
| hotstart | |
| input | purified RNA |
| Quality Level | 300  |
| shipped in | wet ice |
| storage temp. | −20°C |
| technique(s) | RT-qPCR: suitable |
| usage | sufficient for 250 reactions |
| Application: | Quantitative RT-PCR ReadyMix™ has been used in the quantification of messenger RNA (mRNA) levels of specific expressed genes by quantitative reverse-transcription polymerase chain reaction (RT-qPCR). |
| Features and Benefits: | • The master mix allows consistency and reproducibility from one reaction to the next • Reduced risk of contamination from multiple pipetting steps • Reduced set-up time as compared to manual or wax Hot Start methods • JumpStart™Taq Polymerase reduces primer-dimer and non-specific product formation • Broad instrument compatibility • Includes a separate ROX reference dye vial for reaction normalization |
| General description: | Quantitative RT-PCR ReadyMix™ combines Moloney Murine Leukemia Virus Reverse Transcriptase (M-MLV RT) and JumpStart™ Taq DNA polymerase in a one-step RT-PCR kit designed for the measurement of gene expression. This convenient 2X master mix includes M-MLV RT, JumpStart™ Taq DNA polymerase, 99% pure deoxynucleotides, buffer, glass passivator, and stabilizers. The JumpStart™ Taq DNA polymerase is an antibody-inactivated hot-start enzyme. Once the reaction temperature reaches 70°C, the DNA polymerase-antibody complex dissociates and Taq DNA polymerase activity is restored. This antibody-enzyme complex allows for easy and convenient set-up with less contamination risk than manual hot-start techniques. This kit has been formulated for fluorogenic hybridization probe-based detection methods. |
| Legal Information: | Eppendorf is a registered trademark of Eppendorf AG |
| Legal Information: | JumpStart is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | ReadyMix is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | This product is for research use only. |
| Packaging: | 1 kit sufficient for 250 reactions at 20 μL each or for 100 reactions at 50 μL each. |
| Symbol |  GHS07 |
| Signal word | Warning |
| Hazard statements | H317 |
| Precautionary statements | P261 - P272 - P280 - P302 + P352 - P333 + P313 - P362 + P364 |
| RIDADR | NONH for all modes of transport |
| Storage Temp. | −20°C |
| UNSPSC | 41106300 |
| Components | Probe Based qRT-PCR ReadyMix 2 X; Moloney Murine Leukemia Viral Reverse Transcriptase (M-MLV RT) 5000 U; 10X PCR Buffer, Optimized for routine PCR with MgCl2 included 1.5 mL/vial; Magnesium chloride solution, PCR Reagent, 25 mM MgCI2 solution for PCR 25 mM; 10X PCR Buffer, Optimized for routine PCR with MgCl2 included 100 X |