RIPA Buffer
SIGMA/R0278
Synonym: Radioimmunoprecipitation assay buffer
MDL Number: MFCD02100484
Product Type: Chemical
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| color | colorless |
| description | protease free |
| form | liquid |
| packaging | pkg of 50 mL |
| pkg of 500 mL | |
| pH | 7.8-8.2 (25 °C, 1 ×) |
| Quality Level | 200  |
| storage temp. | 2-8°C |
| technique(s) | immunoprecipitation (IP): suitable |
| Application: | RIPA Buffer enables efficient cell lysis and protein solubilization while avoiding protein degradation and interference with the proteins′ immunoreactivity and biological activity. RIPA Buffer also results in low background in immunoprecipitation and molecular pull-down assays. Compatible with EZview™ Affinity Gels. |
| Application: | RIPA Buffer has been used as a reagent for cell lysis in the following experimental studies: • Fibroblast-based deep phenotyping of the peripheral tissue, which facilitates the mechanistic disease stratification in sporadic Parkinson′s disease (sPD). • Demonstrating the inhibition of oxidative phosphorylation (OXPHOS), which can activate the mixed lineage kinase domain-like protein (MLKL)-dependent necroptosis in human lung epithelial cells. |
| Features and Benefits: | 1 mL of the RIPA Buffer is used to lyse cells from one 100 mm culture dish (0.5 to 5 * 107 cells) of most adherent mammalian cell lines. |
| Features and Benefits: | Minimizes non-specific protein binding interactions to ensure low background. |
| General description: | Radioimmunoprecipitation assay buffer (RIPA buffer) is a lysis buffer used for rapid, efficient cell lysis and solubilization of proteins from both adherent and suspension cultured mammalian cells. |
| General description: | RIPA (Radio-Immunoprecipitatio |
| Legal Information: | EZview is a trademark of Sigma-Aldrich Co. LLC |
| Legal Information: | IGEPAL is a registered trademark of Solvay |
| Other Notes: | Ready-to-use solution containing 150 mM NaCl, 1.0% IGEPAL® CA-630, 0.5% sodium deoxycholate, 0.1% SDS, 50 mM Tris, pH 8.0. |
| Symbol |   GHS07,GHS09 |
| Signal word | Warning |
| Hazard statements | H319 - H411 |
| Precautionary statements | P264 - P273 - P280 - P305 + P351 + P338 - P337 + P313 - P391 |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | 2-8°C |
| UNSPSC | 12161703 |