RANK Ligand from mouse
SIGMA/R0525 - ≥98% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder, suitable for cell culture
Synonym: Osteoclast differentiation factor (ODF); Osteoprotegerin ligand (OPGL); Receptor activator of NF-κB Ligand (RANKL); TNF-related activation−induced cytokines (TRANCE)
MDL Number: MFCD01867142
Product Type: Chemical
SDS-PAGE RANK Ligand from Mouse (Cat. No. R0525) was assessed on SDS-PAGE.
Bioassay EC50 is defined as the effective concentration of RANK that elicits a 50% attachment factor in cell based bioassay.
| assay | ≥98% (SDS-PAGE) |
| biological source | mouse |
| color | white |
| form | lyophilized powder |
| impurities | <0.1 ng/mg |
| <1 EU/μg | |
| packaging | pkg of 10 μg |
| potency | 10.0-25.0 ng/mL ED50 |
| Quality Level | 200  |
| recombinant | expressed in E. coli |
| storage condition | avoid repeated freeze/thaw cycles |
| storage temp. | −20°C |
| technique(s) | cell culture | mammalian: suitable |
| UniProt accession no. | O35235  |
| Analysis Note: | The bioactivity is determined by its ability to induce osteoclast differentiation of RAW 264.7. The expected ED50 for this effect is ≤10 ng/ml. |
| Application: | RANK ligand from mouse has been used to visualize differentiated osteoclasts and to stimulate osteoclast differentiation. |
| Application: | The functions of RANK Ligand include induction of activation of the c-jun N-terminal kinase, enhancement of T cell growth and dendritic cell function induction of osteoclastogenesis, and lymph node organogenesis.The cell surface signaling receptor of RANK Ligand is RANK, which undergoes receptor clustering during signal transduction. |
| Biochem/physiol Actions: | Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or RANKL has an important role in osteoclast function and differentiation. It binds to RANK and osteoprotegrin (OPG). Bioactivities of RANKL may be attributed to its binding to these two receptor molecules. Mouse and human RANKL share 85% amino acid sequence identity. |
| General description: | Tumor necrosis factor (ligand) superfamily, member 11 (Tnfsf11) or receptor activator of nuclear factor-κB ligand (RANKL) is a type II transmembrane signaling receptor. It has a molecular weight of about 35kDa. The protein possesses an amino-terminal intracellular tail and a carboxy-terminal extracellular region, which has a connecting stalk and a receptor-binding domain. |
| Physical form: | Lyophilized from a 0.2 μm filtered solution of 5 mM sodium phosphate, pH 7.6, and 75 mM NaCl. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 2 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Purity | ≥98% (SDS-PAGE) |
| Storage Temp. | −20°C |
| UNSPSC | 12352202 |