Anti-Ribosomal Protein L26 (N-terminal) antibody produced in rabbit
SIGMA/R0655 - ~1 mg/mL, affinity isolated antibody, buffered aqueous solution
Synonym: Anti-RPL26
MDL Number: MFCD09265375
Product Type: Chemical
Immunofluorescence Cytoplasmic localization of Ribosomal Protein L26 in HeLa cells stained with Rabbit Anti-Ribosomal Protein L26 (N-terminal), Affinity Isolated Antibody (Cat. No. R0655) at a 5 μg/mL concentration (A), and DAPI (B). The antibody was developed with Goat Anti-Rabbit IgG, Cy3 conjugated.
Immunoblotting The extracts were separated on SDA-PAGE and blotted with Rabbit Anti-Ribosomal Protein L26 (N-terminal) affinity isolated antibody (Cat. No. R0655), at a concentration of 0.25 μg/mL. The antibodies were developed with Goat Anti-rabbit IgG, Peroxidase conjugate (Cat. No. A0545) and a chemiluminiscence substrate. Lysates from the following cell lines were loaded: Lane 1, 293T; Lane 2, HeLa; Lane 3, U2OS; Lane 4, MCF7; Lane 5, K562; Lane 6, A431; Lane 7, Jurkat; Lane 8, HepG2; Lane 9, NIH3T3; Lane 10, PC12; Lane 11, RAT1.
| antibody form | affinity isolated antibody |
| antibody product type | primary antibodies |
| biological source | rabbit |
| clone | polyclonal |
| concentration | ~1 mg/mL |
| conjugate | unconjugated |
| form | buffered aqueous solution |
| mol wt | antigen 17 kDa |
| Quality Level | 200  |
| shipped in | dry ice |
| species reactivity | mouse, human, rat |
| storage temp. | −20°C |
| target post-translational modification | unmodified |
| technique(s) | immunoprecipitation (IP): 5-10 μg using HEK-293T cell lysates |
| indirect immunofluorescence: 5-10 μg/mL using paraformaldehye-fixed HeLa cells | |
| western blot: 0.5-1 μg/mL using HEK-293T cell extracts | |
| UniProt accession no. | P61254  |
| Application: | Anti-Ribosomal Protein L26 (N-terminal) antibody produced in rabbit has been used in: • immunoblotting • immunoprecipitation • immunostaining • immunofluorescence • coimmunoprecipitation analysis |
| Biochem/physiol Actions: | Anti-Ribosomal Protein L26 (N-terminal) specificallyrecognizes human Ribosomal Protein L26. |
| Biochem/physiol Actions: | Ribosomal proteins are implicated in mRNA recognition, tRNA recognition and decoding and nuclear export. It was shown together with nucleolin to bind to the 5′ untranslated region of p53 mRNA and to control p53 translation and induction after DNA damage. |
| Biochem/physiol Actions: | RPL26 (ribosomal protein L26) regulates the expression of p73. It plays an important role in translation and stability of ribosomes. RPL26 frameshift mutation can cause several physical abnormalities. |
| Disclaimer: | Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals. |
| General description: | Ribosomal protein L26 is one of the large ribosomal subunit proteins. |
| General description: | RPL26 (ribosomal protein L26) is located on chromosome 17. It codes for a 145-amino-acid long protein and has four exons. It is present on the large ribosomal subunit. |
| Immunogen: | synthetic peptide corresponding to amino acids 1-17 of human ribosomal protein L26, conjugated to KLH via a C-terminal added cysteine residue. The sequence is conserved in human, rat, and mouse. |
| Physical form: | Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide. |
| RIDADR | NONH for all modes of transport |
| WGK Germany | WGK 3 |
| Flash Point(F) | Not applicable |
| Flash Point(C) | Not applicable |
| Storage Temp. | −20°C |
| UNSPSC | 12352203 |